Kutheni i-cryovials iqhuma?Indlela yokuphepha ngayo?

Kutheni i-cryovials iqhuma?Indlela yokuphepha ngayo?

Ngexesha lovavanyo, sinokusebenzisai-cryovialsukukhenkceza iisampuli, kodwa xa umkhenkce ngenitrogen engamanzi,i-cryovialszihlala ziqhuma, ezingabangeli kuphela ilahleko yeisampulu zovavanyo, kodwa zinokubangela umonakalo kwiisampulu.Abavavanyi benza ingozi, ngoko ke ungayinqanda njani le nto ingenzeki?

Izizathu:

Okokuqala,i-cryovialsayinakubekwa ngokuthe ngqo kwisigaba solwelo senitrogen yolwelo ukuze igcinwe.Ngenxa yokuba izinto eziphathekayo zombhobho kunye ne-cap yesiqheloi-cryovialsahlukile, amaxabiso okwanda kwe-thermal kunye nokucutheka ngexesha lokukhenkceza nawo ahlukile.Ukuba ubeka ii-cryovialngokuthe ngqo kwisigaba solwelo, unokuvumela initrogen engamanzi ukuba ihambe kwityhubhu.Xa uvuselela isampuli kwixesha elizayo, beka iii-cryogenic-vialskwibhafu yamanzi kwi-37 ° C, i-nitrogen engamanzi kwi-tube ngokukhawuleza yanyuka kwaye yanda, kodwa igesi ayikwazanga ukubaleka kwi-tube ngexesha, nto leyo eyabangela ukuba ityhubhu ye-cryopreservation iqhume.

Indlela yokuphepha:

1. Musa ukugcinai-cryovialsngokuthe ngqo kwisigaba solwelo, kodwa kwisigaba segesi.Okanye nje ukhenkceke ngokuthe ngqo kwifriji.Khumbula ukuba ungayibeki ngokuthe ngqo ngaphantsi komphezulu wenitrogen engamanzi.

2. Sebenzisa ukujikeleza kwangaphakathiiicryotubes.

Kakade ke, kwanangaphakathi ejikeleziswayoiicryotubesayinakubekwa ngokuthe ngqo kwisigaba solwelo, kodwa ijikelezwe ngaphakathiiicryotubesbabenokunyamezela ubushushu obuphantsi kuneminqwazi ejikeleziswayo ngaphandle, enokuthi yehlise ugqabhuko-dubulo olunokwenzeka kwaye ikhuseleke noko.Ujikelezo lwangaphandleikriyotubeeneneni yenzelwe ukukhenkceza koomatshini, ngoko ke ayifanelekanga kugcino lwenitrogen engamanzi.

3. Kufuneka wenze ntoni ke ukuba ufuna ngokwenene ukuyigcina kwisigaba solwelo?Ukuphendula kule ngxaki, eneneni kukho imikhono yetyhubhu ye-cryopreservation eyenzelwe ngokukodwa le njongo, enokuthi isetyenziswe ukutywina ityhubhu ye-cryopreservation kwaye emva koko uyibeke kwisigaba solwelo.Ngokuqinisekileyo, ungasebenzisa kwakhona ifilimu yokutywina, i-tape yonyango, njl.njl ukutywina, ukwenzela ukuba kungabikho kuqhuma.