I-PCR 8-strip centrifuge isebenzisa i-centrifugal force enamandla eyenziwa yi-high-speed rotation ye-rotor ye-centrifuge ukuphucula isantya sokumisa iincinci kumanzi kunye nezinto ezihlukeneyo ezinemilinganiselo eyahlukeneyo yokumisa kunye ne-buoyancy kwisampuli.Kuvavanyo lwethu lwebhayoloji, sihlala sisebenzisa i-PCR 8-strip.Ziziphi iinkcukacha ekufuneka ziqwalaselwe ngexesha lokusetyenziswa kwe-PCR 8-strip ?Okulandelayo, iLabio biological iya kuyichaza.
1. Musa ukusebenzisa ngaphandle koqeqesho kunye novavanyo.
2. Khetha i-rotor efanelekileyo kunye nesantya.Okanye, musa ukusebenzisa.
3. Khetha isinyibilikisi sendalo esifanelekileyo ngaphezu kweqondo lobushushu (ngokubanzi yi-4 ℃) ukunqanda umonakalo womkhenkce
1. Musa ukusebenzisa ngaphandle koqeqesho kunye novavanyo.
2. Khetha i-rotor efanelekileyo kunye nesantya.Musa ukusebenzisa.
3. Khetha isinyibilikisi sendalo esifanelekileyo ngaphezu kweqondo lobushushu (ngokubanzi yi-4 ℃) ukunqanda umonakalo womkhenkce
1. Musa ukusebenzisa ngaphandle koqeqesho kunye novavanyo.
2. Khetha i-rotor efanelekileyo kunye nesantya.Musa ukusebenzisa.
3. Khetha i-solvent efanelekileyo ye-organic ngaphezu kweqondo lokushisa (ngokubanzi i-4 ℃) ukukhusela umonakalo wokukhenkceza kwi-PCR 8-strip kunye ne-rotor.
4. Ngaphambi kokusebenzisa i-rotor, sula umbhobho ngentonga yomngxuma kwaye uhlolisise ngokucophelela umngxuma ongezantsi wokuqhekeka okanye amabala amhlophe.Ukuba kunjalo, jonga ngasemva uze uyilahle.
5. Ubungakanani besisombululo kwi-PCR 8-strip kufuneka ifaneleke.Umbhobho wensimbi ongenasici awunasigqubuthelo, kuphela i-2/3 inokugqithwa.Umbhobho weplastiki unokulungelelaniswa negxalaba.Isigqubuthelo kufuneka sivalwe ngaphandle kokuvuza.Ukusetyenziswa kwe-solvents ye-organic kwimibhobho yeplastiki kufuneka ihambelane nemimiselo.
6. Ii-Centrifuges ziya kufakwa kwizibini kwaye zilungelelaniswe kakuhle kunye nokutenxa <0.1g.
7. Awukwazi ukujikeleza ngaphandle kokujikeleza.Ukuphatha kunye ne-rotor, kufuneka sisebenzise isibambo ukuthintela i-rotor ukuba ingatyi.Jika isibane kwaye usivumele ukuba sizinze.Xa ujika isiphatho, bamba isiphatho ngesandla kwaye ujike ikepusi.Isigqubuthelo se-cap ejikelezayo kufuneka sigqunywe ngokuqinileyo.Umnqwazi akufuneki ubekwe phakathi.
8. Musa ukuvula isiciko ngexesha le-centrifugation.I-Centrifuge ayixhaswanga.
9. Emva kokuba i-rotor isetyenziswe, i-rotor iya kususwa ngexesha, yomiswe kwi-rotor cavity, kwaye imingxuma kumbhobho iya kuhlanjululwa ngokucophelela ngentonga yokucoca.Ukuba isisombululo sigqithise, sihlambe sicoceke, sula amanzi adibeneyo kwi-rotor cavity, kwaye uvule umnyango ukuze umise i-rotor cavity.
10. I-centrifuge mayigcinwe ngokufanelekileyo kwaye ibhaliswe kunye nee-manifolds ezisibhozo emva kokusetyenziswa.
kunye ne-rotor.
4. Ngaphambi kokusebenzisa i-rotor, sula umbhobho ngentonga yomngxuma kwaye uhlolisise ngokucophelela umngxuma ongezantsi wokuqhekeka okanye amabala amhlophe.Ukuba kunjalo, jonga ngasemva uze uyilahle.
5. Ubungakanani besisombululo kwi-octuple kufuneka ifaneleke.Umbhobho wensimbi ongenasici awunasigqubuthelo, kuphela i-2/3 inokugqithwa.Umbhobho weplastiki unokulungelelaniswa negxalaba.Isigqubuthelo kufuneka sivalwe ngaphandle kokuvuza.Ukusetyenziswa kwe-solvents ye-organic kwimibhobho yeplastiki kufuneka ihambelane nemimiselo.
6. Ii-Centrifuges ziya kufakwa kwizibini kwaye zilungelelaniswe kakuhle kunye nokutenxa <0.1g.
7. Awukwazi ukujikeleza ngaphandle kokujikeleza.Ukuphatha kunye ne-rotor, kufuneka sisebenzise isibambo ukuthintela i-rotor ukuba ingatyi.Jika isibane kwaye usivumele ukuba sizinze.Xa ujika isiphatho, bamba isiphatho ngesandla kwaye ujike intloko.Intloko ejikelezayo kufuneka igqunywe ngokuqinileyo.Isivalo akufuneki sibekwe kwindawo esembindini.
8. Musa ukuvula isiciko ngexesha le-centrifugation.I-Centrifuge ayixhaswanga.
9. Emva kokuba i-rotor isetyenziswe, i-rotor iya kususwa ngexesha, yomiswe kwi-rotor cavity, kwaye imingxuma kumbhobho iya kuhlanjululwa ngokucophelela ngentonga yokucoca.Ukuba isisombululo sigqithise, sihlambe sicoceke, sula amanzi adibeneyo kwi-rotor cavity, kwaye uvule umnyango ukuze umise i-rotor cavity.
10. I-centrifuge mayigcinwe ngokufanelekileyo kwaye ibhaliswe kunye nee-manifolds ezisibhozo emva kokusetyenziswa.
kunye ne-rotor.
4. Ngaphambi kokusebenzisa i-rotor, sula umbhobho ngentonga yomngxuma kwaye uhlolisise ngokucophelela umngxuma ongezantsi wokuqhekeka okanye amabala amhlophe.Ukuba kunjalo, jonga ngasemva uze uyilahle.
5. Ubungakanani besisombululo kwi-octuple kufuneka ifaneleke.Umbhobho wensimbi ongenasici awunasigqubuthelo, kuphela i-2/3 inokugqithwa.Umbhobho weplastiki unokulungelelaniswa negxalaba.Isigqubuthelo kufuneka sivalwe ngaphandle kokuvuza.Ukusetyenziswa kwe-solvents ye-organic kwimibhobho yeplastiki kufuneka ihambelane nemimiselo.
6. Ii-Centrifuges ziya kufakwa kwizibini kwaye zilungelelaniswe kakuhle kunye nokutenxa <0.1g.
7. Awukwazi ukujikeleza ngaphandle kokujikeleza.Ukuphatha kunye ne-rotor, kufuneka sisebenzise isibambo ukuthintela i-rotor ukuba ingatyi.Jika isibane kwaye usivumele ukuba sizinze.Xa ujika isiphatho, bamba isiphatho ngesandla kwaye ujike intloko.Intloko ejikelezayo kufuneka igqunywe ngokuqinileyo.Isivalo akufuneki sibekwe kwindawo esembindini.
8. Musa ukuvula isiciko ngexesha le-centrifugation.I-Centrifuge ayixhaswanga.
9. Emva kokuba i-rotor isetyenziswe, i-rotor iya kususwa ngexesha, yomiswe kwi-rotor cavity, kwaye imingxuma kumbhobho iya kuhlanjululwa ngokucophelela ngentonga yokucoca.Ukuba isisombululo sigqithise, sihlambe sicoceke, sula amanzi adibeneyo kwi-rotor cavity, kwaye uvule umnyango ukuze umise i-rotor cavity.
10. I-centrifuge kufuneka igcinwe ngokufanelekileyo kwaye ibhaliswe kunye ne-PCR 8-strip emva kokusetyenziswa.
Ixesha lokuposa: Sep-14-2022