Ukukhuliswa kweeseli ngumcimbi wokuhlatywa kwentliziyo kunye nemiphunga.Ufanele uyiphathe ngononophelo njengomntwana, umthande kwaye umkhathalele.Ukuba unikela ingqalelo kwezi ngxaki xa uzinyamekela, iiseli zakho ziya kondliwa ngcono.Ngoku makhe sithethe malunga nezilumkiso zenkcubeko yeeseli.
Ukulungiselela phambi kwenkcubeko yeseli
Ngaphambi kokuba unxibe iiglavu ukuqalisa inkcubeko yeeseli, jonga ukuba inani leepayipi kunye neebhotile zanele, ukuze ugweme ukungena kunye nokushiya ikhonsoli kwakhona emva kovavanyo, olunokunciphisa umngcipheko wongcoliseko lweeseli.
Indawo yenkcubeko yeseli kufuneka ifudunyezwe kuqala.Ukukhetha ukutshisa kuphela indawo ephakathi kunokuba ibhotile yonke ayikwazi nje ukugcina ixesha lokulinga, kodwa kwakhona ukuphepha ukuthotywa kweprotheni okubangelwa ukufudumeza ngokuphindaphindiweyo okuphakathi.
Emva kotyando, ungalibali ukuba i-medium inobuntununtunu ekukhanyeni kwaye kufuneka igcinwe kude nokukhanya kangangoko kunokwenzeka.
Ukuhlolwa rhoqo kwenkcubeko yeeseli
Ukuhlolwa rhoqo kwe-morphology yeeseli ezikhuliswe, oko kukuthi, imilo kunye nenkangeleko, ibalulekile kwimpumelelo yovavanyo lwenkcubeko yeseli.
Ukongeza ekuqinisekiseni imeko esempilweni yeeseli, ukuphonononga iiseli ngamehlo kunye ne-microscope ngalo lonke ixesha usebenzisa iiseli zinokufumana iimpawu zongcoliseko kwangethuba, ukuze kuthintelwe ukusasazeka kongcoliseko kwezinye iiseli elabhoratri.
Iimpawu zokuncipha kweeseli
Iimpawu zokuncipha kweeseli ziquka ukubonakala kweegranules ezijikeleze i-nucleus, ukuchithwa kweeseli ezivela kwi-matrix, kunye nokwakhiwa kwe-cytoplasmic vacuoles.
Ezi mpawu zemetamorphic zinokubangelwa zizizathu ezininzi, ezifana nezi:
Ungcoliseko lwenkcubeko, ukuthomalalisa komgca weeseli, okanye ubukho bezinto eziyityhefu kwindlela yenkcubeko, okanye ezi mpawu zibonisa kuphela ukuba inkcubeko kufuneka itshintshwe.
Xa i-metamorphism inzulu, iya kuba lutshintsho olungenakuguqulwa.
Ukubulala iintsholongwane kunye noyilo lwe-cell culture fume hood
Gcina ihood yenkcubeko yeseli icocekile kwaye icwangciswe, kwaye ubeke zonke izinto ngaphakathi koluhlu lwembono ethe ngqo.
Tshiza i-ethanol engama-70% kuwo onke amanqaku afakwe kwihood yomsi, wosule kwaye uwacoce ukuze abulawe iintsholongwane.
Beka isitya senkcubeko yeseli kwindawo evulekileyo embindini we-fume hood;I-pipette ifakwe kwi-right front for access easy;I-reagent kunye nenkcubeko medium zibekwe ngasemva ekunene ukuze kufunxeka lula;I-tube rack yokuvavanya ihlelwe kwindawo ephakathi ngasemva;Isikhongozeli esincinane sibekwe ngasemva ekhohlo ukuze sibambe ulwelo olulahlwayo.
Ixesha lokuposa: Nov-18-2022