Sebenzisa iNdlela kunye neZilumkiso zeMibhobho yokuBanda
Kwiimvavanyo ze-microbiological, esinye isixhobo sovavanyo sisoloko sisetyenziswa, oko kukuthi, ityhubhu ye-cryopreservation.Nangona kunjalo, ngenxa yokuntsonkotha kwazo okwahlukileyo, iziphumo ziyahluka kakhulu.Ngenxa yesi sizathu, okwangoku, iilabhoratri ezininzi e-China zenza iibhubhu zokugcina iibhaktheriya ngokwazo, ezingagcini nje ukwandisa ukuqina komsebenzi, kodwa nangenxa yokulinganiselwa kweemeko ezahlukeneyo, umphumo wokugcinwa kweebhaktheriya akusoloko ukwanelisa.
Ngoko ke, kuyimfuneko ukulawula indlela yokusetyenziswa kunye nezinye izilumkiso zetyhubhu ye-cryopreservation, ukuze udlale indima enkulu.
1.Indlela yokusetyenziswa
1).Xa usebenzisa ityhubhu ye-cryopreservation ukugcina iisampulu, kufunwa ngokungqongqo ukubeka ityhubhu ye-cryopreservation kumaleko womphunga wenitrogen engamanzi okanye ifriji yokugcina.Ukuba ityhubhu ye-cryopreservation igcinwe kwi-nitrogen engamanzi, kukho ithuba elithile lokuba i-nitrogen engamanzi iya kungena kwi-tube ye-cryopreservation.Ngethuba lokubuyisela, i-gasification ye-nitrogen ye-liquid iya kukhokelela ekungalingani kwengcinezelo yangaphakathi nangaphandle, enokuthi ibangele ukuba ityhubhu ye-cryopreservation iqhume, kwaye ibe neengozi zebhayoloji.
2).Sebenzisa ityhubhu ye-cryopreservation ukuvuselela, kwaye usebenzise izixhobo zokukhusela ukhuseleko kuyo yonke inkqubo.Kunconywa ukuba unxibe iimpahla zebhubhoratri, iiglavu zekotoni kwaye usebenze kwibhentshi yebhubhoratri ekhuselekileyo.Ukuba kunokwenzeka, nceda unxibe iglavu okanye isikhuselo sobuso.Njengoko ubushushu bangaphakathi ehlotyeni buya kuba phezulu kunobusika, nceda uqaphele.
3).Ngexesha lokugcinwa kweeseli ze-cryopreserved, ukushisa okubandayo kweetyhubhu ze-cryopreserved kufuneka kufane.Umkhenkce ongalinganiyo uya kukhokelela kwijem yomkhenkce, eya kuthintela ukuhanjiswa kobushushu bolwelo kumacala omabini, ngaloo ndlela ivelisa uxinzelelo oluphezulu oluyingozi kwaye ibangele umonakalo kumbhobho wokukhenkceza.
4).Ubungakanani beesampulu ezikhenkcezisiweyo aziyi kudlula umthamo omkhulu wokusebenza ofunwa ngumbhobho womkhenkce.
2. Imiba efuna ingqalelo
1).Indawo yokugcina ityhubhu ekhenkcezayo
Iityhubhu ze-cryopreservation ezingasetyenziswanga zingagcinwa kwindawo yobushushu begumbi okanye i-2-8 ℃ kangangeenyanga ezili-12;Ityhubhu ye-cryopreservation ene-inoculated ingagcinwa kwi--20 ℃ kwaye ibe nefuthe elihle lokugcinwa koxinzelelo kwiinyanga ezili-12;Ityhubhu ye-cryopreservation ene-inoculated inokugcinwa kwi-80 ℃, kwaye ubunzima bunokugcinwa kakuhle kwiinyanga ezingama-24.
2).Ixesha lokugcina ityhubhu yokukhenkceza
Iityhubhu ze-cryopreservation ezingasetyenziswanga zingagcinwa kwindawo yobushushu okanye i-2-8 ℃;Ityhubhu ye-cryopreservation ene-inoculated iya kugcinwa ku--20 ℃ okanye - 80 ℃.
3).Amanyathelo okusebenza kwityhubhu yokukhenkcela
Thatha amasiko amatsha kwiinkcubeko zebhaktheriya ezicocekileyo ukulungiselela ukumiswa kwebhaktheriya kunye ne-turbidity malunga ne-3-4 ye-McDonnell's ratio ye-inoculation kunye ne-strain preservation tube;Qinisa ityhubhu yogcino kwaye uyibuyisele ngasemva nangaphambili amaxesha angama-4-5 ukwenza ibhaktheriya i-emulsified, ngaphandle kokujikeleza;Faka ityhubhu yogcino efrijini ukuze ugcinwe (- 20 ℃ – 70 ℃
Ixesha lokuposa: Nov-25-2022