Iingcebiso ekukhetheni nasekusetyenzisweni kweepleyiti zenkcubeko yeeseli (I)
Njengesixhobo esiqhelekileyo nesibalulekileyo senkcubeko yeeseli, ipleyiti yenkcubeko yeeseli inemilo eyahlukeneyo, ingcaciso kunye nokusetyenziswa.
Ngaba nawe ubhidekile malunga nendlela yokukhetha ipleyiti yenkcubeko efanelekileyo?
Ngaba unexhala malunga nendlela yokusebenzisa ipleyiti yenkcubeko ngokulula nangokuchanekileyo?
Ngaba udidekile malunga nendlela yokujongana nepleyiti yenkcubeko?
Uziva njani ngokusetyenziswa okumangalisayo kwepleyiti yeenkcubeko ezahlukeneyo?
Ungayikhetha njani ipleyiti yenkcubeko yeseli?
1) Iipleyiti zenkcubeko yeseli zinokwahlulwa zibe sezantsi tyaba kunye nezantsi ezantsi (U-shaped kunye no-V-shaped) ngokwemilo yezantsi;
2) Inani lemingxuma yenkcubeko yayiyi-6, 12, 24, 48, 96, 384, 1536, njl.
3) Ngokwezixhobo ezahlukeneyo, kukho ipleyiti yeTerasaki kunye nepleyiti yenkcubeko yeseli eqhelekileyo.Ukhetho oluthile luxhomekeke kuhlobo lweeseli ezikhuliswe, umthamo wenkcubeko ofunekayo kunye neenjongo ezahlukeneyo zokulinga.
Umahluko kunye nokukhethwa kweeplati zenkcubeko ezisicaba kunye nezijikelezayo (ezibunjwe ngu-U kunye no-V).
Iindidi ezahlukeneyo zeebhodi ngokwendalo zineendlela ezahlukeneyo zokusetyenziswa
Zonke iintlobo zeeseli ezisezantsi zinokusetyenziswa, kodwa xa inani leeseli lincinci, njenge-cloning, kusetyenziswa iipleyiti ezisezantsi ezimcaba ezingama-96.
Ukongeza, xa usenza i-MTT kunye nezinye iimvavanyo, ipleyiti esezantsi esezantsi isetyenziswa ngokubanzi kuzo zombini iiseli ezibambelelayo kunye nezixhonyiweyo.
Ngokubhekiselele kwiipleyiti ezinobumba obu-U okanye obu-V, zisetyenziswa ngokubanzi kwiimfuno ezikhethekileyo.Ngokomzekelo, kwi-immunology, xa i-lymphocyte ezimbini ezahlukeneyo zixutywe, kufuneka zidibanise ukuze zivuselele.Ngoko ke, iipleyiti ezibunjwe ngu-U ziyafuneka ngokubanzi.Ngenxa yokuba iiseli ziya kuqokelelana kuluhlu oluncinci ngenxa yempembelelo yomxhuzulane, iipleyiti ezinobumba obu-V aziluncedo kangako.Iipleyiti ezinokwakheka kwe-V zidla ngokusetyenziswa kwiimfuniselo zokubulala iiseli ukwenza iiseli ekujoliswe kuzo ngokusondeleyo, kodwa iipleyiti ezimilise u-U nazo zingasetyenziswa kolu vavanyo (emva kokongeza iiseli, i-centrifuge ngesantya esiphantsi).
Ukuba isetyenziselwa ukukhuliswa kweeseli, idla ngokuba ngumzantsi osicaba.Ukongezelela, ingqalelo ekhethekileyo kufuneka ihlawulwe kwizinto eziphathekayo.Uphawu "Inkcubeko yeTissue (TC) Iphathwe" isetyenziselwa inkcubeko yeseli.
Umzantsi ojikelezayo udla ngokusetyenziselwa uhlalutyo, ukusabela kweekhemikhali, okanye ukugcinwa kwesampulu.Kuba amazantsi angqukuva angcono ukufunxa ulwelo, kwaye umzantsi osicaba awukho.Nangona kunjalo, ukuba ufuna ukulinganisa ixabiso lokufunxa ukukhanya, kufuneka uthenge esezantsi esicaba.
Uninzi lweeseli zenkcubeko zisebenzisa iipleyiti zenkcubeko ezisicaba, ekulula ukuzijonga phantsi kwemakroskopu, zinendawo esezantsi ecacileyo, ezinobume bomgangatho wolwelo lweseli ngokwentelekiso, kwaye zikwaququzelela ukubhaqwa kwe-MTT.
Ipleyiti yenkcubeko engqukuva esezantsi isetyenziswa ikakhulu kuvavanyo lokubandakanywa kwe-isotopu, kwaye isixhobo sokuqokelela iiseli siyafuneka ukuqokelela inkcubeko yeseli, efana “nenkcubeko ye-lymphocyte exutyiweyo”.
Ixesha lokuposa: Dec-08-2022