IELISAipleyiti sisixhobo esiyimfuneko kwi-ELISA, i-enzyme-edityaniswe ne-immunosorbent assay.Zininzi izinto ezichaphazela impumelelo yovavanyo lwe-ELISA.Ukukhetha isixhobo esifanelekileyo linyathelo lokuqala.Ukukhetha i-microplate efanelekileyo kuya kunceda uvavanyo lube yimpumelelo.
Izinto zeELISAipleyiti ngokubanzi i-polystyrene (PS), kwaye i-polystyrene inozinzo olulambathayo lwekhemikhali kwaye inokunyibilika ngeentlobo ezahlukeneyo zezinyibilikisi zendalo (ezifana neehydrocarbons ezinuka kamnandi, iihalogenated hydrocarbons, njl.njl.), kwaye zinokugqweswa ziiasidi ezomeleleyo kunye nealkali.Ayixhathisi kwigrisi kwaye iguquguquke ngokulula emva kokuvezwa kukukhanya kwe-UV.
Zeziphi iindidi zeELISAiipleyiti zikhona?
✦Khetha ngombala
Ipleyiti ecacileyo:ifanelekile kuvavanyo lovavanyo lomzimba oluluqilima kunye nenqanaba eliqinileyo kunye novavanyo olubophayo;
Ipleyiti emhlophe:ilungele i-self-luminescence kunye ne-chemiluminescence;
Ipleyiti emnyama:ilungele i-fluorescent immunoassays kunye neemvavanyo zokubopha.
✦Khetha ngokubopha amandla
Ipleyiti ebophelelayo ephantsi:Ibophelela ngokuqhubekayo kwiiprotheni ngokusebenzisa iibhondi ze-hydrophobic.Ifanelekile njengomphathi wesigaba esiqinileyo kwiiprotheni ze-macromolecular ezinobunzima be-molecular> 20kD.Umthamo wayo wokubopha iprotheni yi-200 ~ 300ng IgG / cm2.
Ipleyiti yokubopha ephezulu:Emva kokunyanga kwendawo, amandla ayo okubopha iprotheni aphuculwe kakhulu, afikelela kwi-300 ~ 400ng IgG / cm2, kwaye ubunzima bemolekyuli yeprotheni eboshiweyo engundoqo> 10kD.
✦ Hlela ngokwemilo esezantsi
Umzantsi osicaba:isalathisi esisezantsi se-refractive, esilungele ukufunyanwa nabafundi be-microplate;
U ezantsi:Isalathisi se-refractive siphezulu, esilungele ukongeza, ukunqwenela, ukuxuba kunye neminye imisebenzi.Unokujonga ngokuthe ngqo utshintsho lombala ngokuhlola okubonakalayo ngaphandle kokuyibeka kwisifundi se-microplate ukugqiba ukuba kukho ukusabela okuhambelana nokuzivikela komzimba.
Ixesha lokuposa: Dec-22-2023