Amanyathelo athile enkcubeko yeSeli

1. Izixhobo eziqhelekileyo

1. Izixhobo kwigumbi lokulungiselela

Isithambiso samanzi adiyiweyo esinye, idisti yamanzi adiyiweyo kabini, itanki yeasidi, i-oveni, i-pressure cooker, ikhabhathi yokugcina (ukugcina amanqaku angenazintsholongwane), ikhabhathi yokugcina (ukugcina amanqaku antsholongwane), itafile yokupakisha.Izixhobo kwigumbi lokulungiselela isisombululo: ibhalansi ye-torsion kunye nebhalansi ye-elektroniki (iyeza lokulinganisa), imitha ye-PH (ukulinganisa ixabiso le-PH yesisombululo senkcubeko), i-magnetic stirrer (ukuqwalasela igumbi lesisombululo sokuvuselela isisombululo).

2. Izixhobo zegumbi lenkcubeko

Itanki ye-nitrogen yolwelo, ikhabhinethi yokugcina (i-sundries yokugcina), isibane se-fluorescent kunye nesibane se-ultraviolet, inkqubo yokucoca umoya, ifriji yokushisa ephantsi (- 80 ℃), i-air conditioner, i-carbon dioxide cylinder, itafile yecala (iirekhodi zokubhala zovavanyo).

3. Izixhobo emazibekwe kwigumbi elingenazintsholongwane

I-Centrifuge (ukuqokelela iiseli), i-ultra-clean worktable, i-microscope eguquliweyo, i-CO2 incubator (inkcubeko yokufukamela), ukuhlamba amanzi, i-disinfection ye-oksijini emithathu kunye nomshini wokubulala inzala, i-4 ℃ ifriji (ibeka i-serum kunye nesisombululo senkcubeko).

2. Ukusebenza kweAseptic

(1) Ukuvalwa kwegumbi elingenazintsholongwane

1. Coca igumbi eliyinyumba rhoqo: kanye ngeveki, sebenzisa kuqala amanzi okupompoza ukugoqa umgangatho, usule itafile, kwaye ucoce itafile yokusebenza, kwaye usebenzise i-3 ‰ lysol okanye i-bromogeramine okanye i-0.5% ye-peracetic acid ukusula.

2. Sterilization of CO2 incubator (incubator): kuqala usule nge 3 ‰ bromogeramine, uze usule nge 75% yotywala okanye 0.5% peracetic acid, kwaye ke irradiate ngesibane ultraviolet.

3. Ukuvala inzalo phambi kovavanyo: vula isibane se-ultraviolet, i-sterilizer ye-oxygen emithathu kunye nenkqubo yokucoca umoya kwi-20-30 imizuzu ngokulandelanayo.

4. Ukuvala inzala emva kovavanyo: sula itafile ecocekileyo, itafile yecala kunye nenqanaba le-microscope eguqulelwe nge-75% yotywala (3 ‰ bromogeramine).

Ukulungiswa kokuvala inzala kwabasebenzi basebhubhoratri

1. Hlamba izandla ngesepha.

2. Nxiba iimpahla zodwa, iikepusi zokuzibekela bucala, iimaski kunye neziliphu.

3. Sula izandla nge-75% yebhola yomqhaphu yotywala.

Umboniso womsebenzi onyumba

1. Zonke iibhotile zotywala, i-PBS, i-culture medium kunye ne-trypsin eziswe kwi-ultra-clean workbench kufuneka zosulwe nge-75% yotywala kumphezulu ongaphandle webhotile.

2. Sebenza kufutshane nedangatye lesibane sotywala.

3. Izitya kufuneka zicocwe phambi kokusetyenziswa.

4. Izinto ezisetyenziswayo (ezifana neminqwazi yeebhotile needropha) eziqhubeka zisetyenziswa kufuneka zibekwe kwindawo ephakamileyo, kwaye zifanele ukuba zisatshiswe ngokugqithisileyo xa zisetyenziswa.

5. Yonke imisebenzi kufuneka isondele kwisibane sotywala, kwaye isenzo kufuneka sibe lula kwaye sichaneke, kwaye akufanele sichukunyiswe ngokungaqhelekanga.Ukuba umququ awunakuchukumisa itanki yolwelo olulahlwayo.

6. Xa unqwenela ngaphezulu kweentlobo ezimbini zolwelo, nikela ingqalelo ekutshintsheni umbhobho wokufunxa ukuthintela ukungcoliseka komnqamlezo.

Jonga kwisahluko esilandelayo sokubulala iintsholongwane kwizixhobo zokusebenza.