Iipleyiti zenkcubeko yeseli zinokwahlulwa zibe sezantsi tyaba kunye nezantsi ezingqukuva (U-shaped kunye no-V-shaped) ngokwemilo yezantsi;Inani lemingxuma yenkcubeko yayiyi-6, 12, 24, 48, 96, 384, 1536, njl.Ngokwezixhobo ezahlukeneyo, kukho ipleyiti yeTerasaki kunye nepleyiti yenkcubeko yeseli eqhelekileyo.Ukhetho oluthile luxhomekeke kuhlobo lweeseli ezikhuliswe, umthamo wenkcubeko ofunekayo kunye neenjongo ezahlukeneyo zokulinga.
(1) Umahluko kunye nokukhethwa kweepleyiti zenkcubeko ezisicaba kunye nezingqukuva (ezimilise u-U kunye no-V).
Iimilo ezahlukeneyo zeepleyiti zenkcubeko zinosetyenziso olwahlukileyo.Iiseli zenkcubeko zihlala zimcaba, nto leyo ilungele ukujongwa nge-microscopic, enendawo esezantsi ecacileyo kunye nomgangatho wolwelo wenkcubeko yeseli.Ngoko ke, xa wenza i-MTT kunye nezinye iimvavanyo, i-flat flat plate isetyenziswa ngokubanzi, kungakhathaliseki ukuba iiseli zifakwe eludongeni okanye zimisiwe.Ipleyiti enomzantsi osicaba kufuneka isetyenziswe ukulinganisa ixabiso lokufunxa.Nika ingqwalasela ekhethekileyo kwizinto eziphathekayo, kwaye uphawule "iNkcubeko yeTissue (TC) yoNyango" kwinkcubeko yeeseli.
Iipleyiti ezinobumba obungu-U okanye obu-V zisetyenziswa ngokubanzi kwiimfuno ezikhethekileyo.Ngokomzekelo, kwi-immunology, xa i-lymphocyte ezimbini ezahlukeneyo zixutywe kwinkcubeko, kufuneka zidibanise kwaye zivuselelane.Ngeli xesha, iipleyiti ezibunjwe ngu-U zisetyenziswa ngokubanzi kuba iiseli ziya kubuthana kuluhlu oluncinci ngenxa yesiphumo sobunzima.Ipleyiti yenkcubeko engqukuva esezantsi ingasetyenziselwa ukulinga ukufakwa kwe-isotopu, efuna isixhobo sokuqokelela iiseli ukuqokelela inkcubeko yeseli, efana “nenkcubeko ye-lymphocyte exubeneyo”.Iipleyiti ezimile okwe-V zihlala zisetyenziselwa ukubulala iiseli kunye novavanyo lwe-immunological blood agglutination.Uvavanyo lokubulala iiseli lunokutshintshwa kwakhona nge-plate ene-U (emva kokongeza iiseli, i-centrifuge ngesantya esiphantsi).
(2) Umahluko phakathi kwepleyiti yeTerasaki kunye nepleyiti yenkcubeko yeseli eqhelekileyo
Ipleyiti yeTerasaki isetyenziswa ikakhulu kuphando lwecrystallographic.Uyilo lwemveliso lulungele ukubonwa kwekristale kunye nohlalutyo lwesakhiwo.Kukho iindlela ezimbini: ukuhlala kunye nokuxhoma ukuwela.Ezi ndlela zimbini zisebenzisa ubume bemveliso eyahlukeneyo.Ipolymer yeklasi yeCrystal ikhethwe njengemathiriyeli, kwaye izixhobo ezikhethekileyo zilungele ukujonga ubume bekristale.
Ipleyiti yenkcubeko yeseli yenziwa ikakhulu ngezinto zePS, kwaye izinto ziphathwa umphezulu, olungele ukukhula kokubambelela kwiseli kunye nokwandiswa.Ewe kunjalo, kukwakho nemathiriyeli yokukhula kweeseli zeplanktonic, kunye nomgangatho ophantsi wokubopha.
(3) Umahluko phakathi kwepleyiti yenkcubeko yeseli kunye nepleyiti kaElisa
Ipleyiti ye-Elisa ibiza kakhulu kunepleyiti yenkcubeko yeseli.Ipleyiti yeseli isetyenziswa ikakhulu kwinkcubeko yeeseli kwaye ingasetyenziselwa ukulinganisa iprotein concentration;Ipleyiti kaElisa ibandakanya ipleyiti yokwambathisa kunye nepleyiti yokusabela, kwaye ayidingi kusetyenziselwa inkcubeko yeseli.Isetyenziselwa ikakhulu ukuchongwa kweprotheyini emva kokusabela okudityaniswe yi-enzyme, efuna iimfuno eziphezulu kunye nesisombululo esisebenzayo seleyibhile ye-enzyme.
(4) Indawo esezantsi yomngxuma kunye nedosi ecetyiswayo yolwelo yeepleyiti zenkcubeko ezisetyenziswa ngokuqhelekileyo
Inqanaba lolwelo lolwelo lwenkcubeko olongeziweyo kwiipleyiti ze-orifice ezahlukeneyo akufanele lube nzulu kakhulu, ngokubanzi phakathi koluhlu lwe-2 ~ 3mm.Isixa esifanelekileyo solwelo lomngxuma ngamnye wenkcubeko singabalwa ngokudibanisa indawo esezantsi yemingxuma eyahlukeneyo.Ukuba i-liquid eninzi yongezwa, i-gas (i-oksijeni) yotshintshiselwano iya kuchaphazeleka, kwaye kulula ukugqithisa ngexesha lenkqubo yokuhamba, kubangela ukungcola.Ukuxinana kweeseli ezithile kuxhomekeke kwinjongo yovavanyo.
Ixesha lokuposa: Nov-04-2022