Ukuqokelela iisampulu, ukugcinwa kunye neemfuno zothutho kwimifuniselo eqhelekileyo

Ukuqokelela iisampulu, ukugcinwa kunye neemfuno zothutho kwimifuniselo eqhelekileyo

1. Ukuqokelela kunye nokugcinwa kwemizekelo ye-pathological:

☛Icandelo lomkhenkce: Susa iibhloko zeethishu ezifanelekileyo kwaye uzigcine kulwelo lwenitrogen;

☛Ukwahlulahlula iparafini: Susa iibhloko zeethishu ezifanelekileyo kwaye uzigcine kwi-4% yeparaformaldehyde;

☛Izilayidi zeSeli: Izilayidi zeseli zalungiswa kwi-4% paraformaldehyde kangangemizuzu engama-30, emva koko zatshintshwa nge-PBS zaza zantywiliselwa kwi-PBS zaza zagcinwa kwi-4°C.

2. Ukuqokelelwa nokugcinwa kwemizekelo yebhayoloji yemolekyuli:

☛Izicubu ezitsha: Sika umzekelo uze uwugcine kulwelo lwenitrogen okanye -80°C efrijini;

☛Imizekelo yeparafini: Gcina kwiqondo lobushushu legumbi;

☛Umzekelo wegazi elipheleleyo: Thatha isixa esifanelekileyo segazi elipheleleyo kwaye wongeze i-EDTA okanye i-heparin anticoagulation ityhubhu yokuqokelela igazi;

☛Iisampulu zolwelo lomzimba: isantya esiphezulu secentrifugation ukuqokelela intlenga;

☛Iisampulu zeSeli: Iiseli zihlanjululwa ngeTRizol kwaye zigcinwe kulwelo lwenitrogen okanye -80°C efrijini.

3. Ukuqokelelwa nokugcinwa kwemizekelo yovavanyo lweprotheyini:

☛Izicubu ezitsha: Sika umzekelo uze uwugcine kulwelo lwenitrogen okanye -80°C efrijini;

☛Umzekelo wegazi elipheleleyo: Thatha isixa esifanelekileyo segazi elipheleleyo kwaye wongeze i-EDTA okanye i-heparin anticoagulation ityhubhu yokuqokelela igazi;

☛Imizekelo yeeseli: Iiseli zihlanjululwe ngokupheleleyo ngesisombululo seseli lysis kwaye emva koko zigcinwe kulwelo lwenitrogen okanye -80°C efrijini.

4. Ukuqokelela kunye nokugcinwa kwe-ELISA, i-radioimmunoassay, kunye neesampuli zovavanyo lwe-biochemical:

☛Isampulu yeSerum (iplasma): Thatha igazi elipheleleyo kwaye ulifake kwityhubhu ye-procoagulation (ityhubhu ye-anticoagulation), i-centrifuge ku-2500 rpm malunga nemizuzu engama-20, qokelela i-supernatant, kwaye uyigcine kulwelo lwenitrogen okanye kwi- -80°C efrijini;

☛Isampuli yomchamo: i-centrifuge isampuli kwi-2500 rpm malunga nemizuzu engama-20, kwaye uyigcine kwi-nitrogen engamanzi okanye -80 ° C efrijini;bhekisa kule ndlela ye-thoracic kunye ne-ascites fluid, i-cerebrospinal fluid, kunye ne-alveolar lavage fluid;

☛Iisampulu zeSeli: Xa ubhaqa amacandelo afihliweyo, i-centrifuge iisampulu kwi-2500 rpm malunga nemizuzu engama-20 kwaye uzigcine kwinitrogen engamanzi okanye -80°C efrijini;xa ufumanisa iinqununu ze-intracellular, Nciphisa ukumiswa kweeseli kunye ne-PBS kunye neqhwa kwaye udibanise ngokuphindaphindiweyo ukutshabalalisa iiseli kunye nokukhulula i-intracellular components.I-Centrifuge kwi-2500 rpm malunga nemizuzu engama-20 kwaye uqokelele i-supernatant njengenhla;

☛Iisampulu zezicubu: Emva kokusika iisampulu, zilinganise uze uzikhenkce kwinitrogen engamanzi okanye -80°C efrijini ukuze zisetyenziswe kamva.

5. Ingqokelela yomzekelo weMetabolomics:

☛Isampuli yomchamo: I-Centrifuge isampuli kwi-2500 rpm malunga nemizuzu engama-20 kwaye uyigcine kwi-nitrogen engamanzi okanye -80 °C efrijini;bhekisa kule ndlela ye-thoracic kunye ne-ascites fluid, i-cerebrospinal fluid, i-alveolar lavage fluid, njl.;

☛Emva kokusika isampulu yethishu, yikale kwaye uyikhenkce kwinitrogen engamanzi okanye -80°C efrijini ukuze isetyenziswe kamva;