Imiqathango yokusetyenziswa kwezitya zePetri
Ukucocwa kwezitya zikaPetri
1. Ukufunxwa: Lungisa iglasi entsha okanye esetyenzisiweyo ngamanzi acocekileyo ukuthambisa kunye nokunyibilikisa isincamathelisi.Ngaphambi kokusebenzisa i-glassware entsha, yixube ngokulula ngamanzi epompo, kwaye emva koko uyicwilise kwi-5% ye-hydrochloric acid ngobusuku;I-glassware esetyenzisiweyo ihlala iqulethe iiprotheyini ezininzi kunye neoli, okungekho lula ukuxubha emva kokumisa, ngoko kufuneka ifakwe emanzini acocekileyo ngokukhawuleza emva kokusetyenziswa ukuxubha.
2. Ukuxukuxa: faka i-glassware efakwe emanzini kunye ne-brush ngokuphindaphindiweyo ngebrashi ethambileyo.Musa ukushiya iikona ezifileyo kwaye uthintele umonakalo kwisiphelo somphezulu wezikhongozeli.Geza kwaye uyomise i-glassware ecocekileyo yokukhetha.
3. I-Pickling: I-Pickling kukucwina iinqanawa ezingentla kwisisombululo sokucoca, esaziwa njengesisombululo se-asidi, ukususa iintsalela ezinokwenzeka kumphezulu weenqanawa ngokusebenzisa i-oxidation eqinile yesisombululo se-asidi.Ukuvunwa akusayi kuba ngaphantsi kweeyure ezintandathu, ubusuku bonke okanye ngaphezulu.Qaphela xa ubeka kwaye uthatha izitya.
4. Ukugungxulwa: Iinqanawa emva kokuxutywa kunye nokucolwa kufuneka zigungxulwe ngokupheleleyo ngamanzi.Ingaba iinqanawa zihlanjwe zicocekile emva kokucholwa kuchaphazela ngqo impumelelo okanye ukungaphumeleli kwenkcubeko yeseli.Ukuhlamba ngesandla kwiinqanawa ezifakwe emanzini, isitya ngasinye siya kuphinda "sizaliswe ngamanzi - sikhutshwe" ubuncinane ngamaxesha angama-15, kwaye ekugqibeleni sihlanjwe ngamaxesha angama-2-3 ngamanzi aqhumayo, omisiwe okanye omisiwe, kwaye apakishwe ngokulinda.
5. Izitya zeplastiki ezilahlwayo zivalwa ngemitha okanye zicolwe ngekhemikhali xa ziphuma kumzi-mveliso.
Ukuhlelwa kwezitya zePetri
2. Inokwahlulwa ibe yiplastiki yepetri izitya kunye neglasi yepetri izitya ngokwezinto ezahlukeneyo zokuvelisa, kodwa zombini izitya zepetri ezithunyelwa ngaphandle kunye nezitya zepetri ezilahlayo zizinto zeplastiki.
3. Ngokutsho kobukhulu obuhlukeneyo, banokuthi bahlulwe ngokubanzi kwi-35mm, 60mm kunye ne-90mm ububanzi.150mm Petri isitya.
4. Ngokwezahlulo ezahlukeneyo, zinokuhlulwa zibe yi-2 izitya zePetri ezihlukeneyo, izitya ezi-3 ezihlukeneyo zePetri, njl.
5. Izinto ezisetyenziselwa izitya zenkcubeko zihlulwe ngokwesiseko zibe ngamacandelo amabini, ngokukodwa iplastiki kunye neglasi.Iglasi ingasetyenziselwa izinto zezityalo, inkcubeko ye-microbial kunye nenkcubeko yokubambelela kwiiseli zezilwanyana.Izinto zeplastiki zinokuba yi-polyethylene, enokusetyenziswa kanye okanye ngamaxesha amaninzi.Zilungele ukugonywa kwebhubhoratri, ukuloba, kunye nemisebenzi yokwahlula iibhaktheriya, kwaye zingasetyenziselwa ukulima izinto zezityalo.
Imiqathango yokusetyenziswa kwezitya zePetri
1. Isitya senkcubeko siyacocwa kwaye sibulawe iintsholongwane phambi kokusetyenziswa.Ingaba icocekile okanye ayinayo impembelelo enkulu kumsebenzi, onokuchaphazela i-pH yendawo yenkcubeko.Ukuba kukho imichiza ethile, iya kuthintela ukukhula kweebhaktheriya.
2. Izitya zenkcubeko ezisanda kuthengwa kufuneka zihlanjwe ngamanzi ashushu kuqala, emva koko zifakwe kwi-1% okanye i-2% yesisombululo se-hydrochloric acid iiyure eziliqela ukususa izinto ze-alkaline zamahhala, kwaye zihlanjwe kabini ngamanzi adibeneyo.
3. Ukuhlakulela iibhaktheriya, sebenzisa umphunga woxinzelelo oluphezulu (ngokubanzi 6.8 * 10 Pa high pressure steam ukuya kumandla we-5th), yifake inzalo kwi-30min kwi-120 ℃, yomise kwiqondo lokushisa, okanye usebenzise ubushushu obomileyo ukuyicolisa, oko kukuthi, beka isitya senkcubeko kwi-oveni, sigcine i-2h kwi-120 ℃, kwaye emva koko ubulale amazinyo entsholongwane.
4. Izitya zenkcubeko ezisterilized zingasetyenziselwa ukugonywa kunye nokulima.
Ixesha lokuposa: Dec-28-2022