Permeable iingcali inkcubeko cell: Cell Culture Faka

Permeable iingcali inkcubeko cell: Cell Culture Faka

Faka iNkcubeko yeSeli, ekwabizwa ngokuba ziinkxaso ezinokungena, njengoko igama libonisa, kukufakwa kwenkcubeko esetyenziselwa imifuniselo enxulumene nomsebenzi wokungena.Kukho i-membrane ekwazi ukungena ezantsi kwenkcubeko yokufaka kunye ne-micropores yobukhulu obuhlukeneyo.Intsalela yekomityi yenziwe ngezinto ezifanayo njengeplate ye-orifice eqhelekileyo.

Ufakelo lwenkcubeko yeseli luqhele ukusetyenziswa kuvavanyo lweeseli, olufana novavanyo lwenkcubeko yokubambisana, uvavanyo lwe-chemotaxis, uvavanyo lokufuduka kweeseli zethumba, ukuhlasela kweseli yethumba, kunye nokuthuthwa kweeseli..

Phakathi kwazo, inkxaso evunyelekayo inokuphucula ngokufanelekileyo inkcubeko yeeseli zepolar kuba ezi zixhaso zivumela iiseli ukuba zikhuphe kwaye zifunxe iimolekyuli ukusuka kwindawo yazo esisiseko kunye ne-apical, ngaloo ndlela zixutywa ngendlela yendalo ngakumbi kwaye zilinganisa indawo ye-vivo kwinkcubeko ethile imigca yeeseli ezikhethekileyo. .

Ngokutsho kwamacwecwe ahlukeneyo, ukufakwa kwenkcubeko kunokwahlulwa ibe yi-6-well, 12-well, kunye ne-24-well.

Ngokweemitha zepore ezahlukeneyo, zahlulwe zibe yi-0.4μm, 3μm, 5μm kunye ne-8μm ukusuka kwidayamitha encinci yepore ukuya kwidayamitha enkulu.

Uphawu:

• Uyilo oluyilayo lomphetho wokongezwa kwesampulu lula

• I-membrane ye-PC: izinga eliphantsi le-adsorption, ukunciphisa ukulahleka kweeprotheni ezincinci ze-molecular kunye nezinye iikhompawundi

• Ifilimu ye-PET inokukhanya okugqwesileyo kunye nokucaca okubonakalayo okubhetele, okwenza kube lula ukujonga isimo seseli

• Iyahambelana noninzi lwezinyibilikisi nezinyibilikisi

• Ifumaneka ngokobukhulu obahlukeneyo, ingasetyenziswa ne-6-well, 12-well, 24-well culture plates kunye nezitya eziyi-100mm.