Itekhnoloji yenkcubeko yezilwanyana/yesityalo luluhlu lwemisebenzi yebhayoloji eyenziwa kwiiseli zezityalo ezizimeleyo okanye iiprotoplasts kwinqanaba leeselula ngenjongo ethile.Ibandakanya ukubekwa wedwa, inkcubeko, ukuvuselelwa kunye nothotho lwemisebenzi enxulumeneyo.Ngokubhekiselele kwimveliso yeekhompawundi eziluncedo, ibhekisa ikakhulu kwinkqubo yokuvelisa iikhompawundi eziluncedo ngokunqunyanyiswa kwenkcubeko yeeseli zezityalo phantsi kweemeko ezingcolileyo.
Shandong Labioiiseli eziziintsholongwane ezinxantathu shake flask yenzelwe inkcubeko iiseli ukumiswa 293, HH kunye nezinye iiseli.Iflaski yokushukuma inomzantsi osicaba kunye nesigqubuthelo esiphefumlayo.Ingasetyenziswa kuphuhliso lwenkqubo encinci, ukukhulisa inyathelo ngenyathelo kunye nezinye izigaba zenkcubeko.Isigqubuthelo esiphefumulayo sinefilimu ephefumulayo ye-0.2μm, ephefumulayo kwaye ingena emanzini, enokuthintela ngokufanelekileyo ukungena kwe-microorganisms, ukukhusela ukungcola, nokuqinisekisa ukutshintshiselwa kwegesi, ukuvumela iiseli ukuba zikhule kakuhle.
Xa usebenzisa i-triangular shake flask, kungcono ukulawula ubungakanani benkcubeko yenkcubeko eyongezwe kwi-20 ukuya kwi-30% yomthamo weflask.Kukho imigca yokuphumelela ecacileyo kwiflask ye-triangular shake ukuze kube lula ukubhekisela kuyo.Isantya sokujikeleza kwe-shaker sinconywa ukuba silawulwe kwi-75 ~ 125RPM, enokuthi ihlengahlengiswe ngokwemeko yangempela.
Ixesha lokuposa: Dec-21-2023