Intshayelelo kwiMidiya yeNkcubeko yeMicrobial eqhelekileyo (I)

Intshayelelo kwiMidiya yeNkcubeko yeMicrobial eqhelekileyo (I)

Isiphakathi senkcubeko luhlobo lwematrix yezondlo ezixubeneyo ezilungiselelwe ukusuka kwizinto ezahlukeneyo ngokweemfuno zokukhula kwe-microbial ezahlukeneyo, ezisetyenziselwa inkcubeko okanye ukwahlula ii-microorganisms ezahlukeneyo.Ngoko ke, i-matrix yezondlo kufuneka iqulethe izondlo (kubandakanya umthombo wekhabhoni, umthombo we-nitrogen, amandla, ityuwa ye-inorganic, izinto zokukhula) kunye namanzi anokusetyenziswa ngama-microorganisms.Ngokuxhomekeke kuhlobo lwe-microorganisms kunye nenjongo yovavanyo, kukho iintlobo ezahlukeneyo kunye neendlela zokulungiselela imidiya yenkcubeko.

Eminye imidiya yenkcubeko eqhelekileyo kuvavanyo yaziswa ngolu hlobo lulandelayo:

I-agar medium yesondlo:

I-agar medium yezondlo isetyenziselwa ukusasaza kunye nenkcubeko yeebhaktheriya eziqhelekileyo, ukuzimisela ukubalwa kwebhaktheriya epheleleyo, ukugcinwa kweentlobo zebhaktheriya kunye nenkcubeko ecocekileyo.Izithako eziphambili zezi: i-extract yenkomo, i-yeast extract, i-peptone, i-sodium chloride, i-agar powder, amanzi adibeneyo.I-Peptone kunye ne-powder yenkomo inika i-nitrogen, i-vitamin, i-amino acid kunye nemithombo ye-carbon, i-sodium chloride inokugcina uxinzelelo lwe-osmotic olulinganayo, kunye ne-agar yi-coagulant ye-culture medium.

I-agar enesondlo lolona hlobo lusisiseko senkcubeko, equlethe uninzi lwezondlo ezifunekayo ekukhuleni kwe-microbial.I-agar yesondlo ingasetyenziselwa inkcubeko yebhaktheriya yesiqhelo.

Blood agar medium:

Igazi leagar medium luhlobo lwenyama yenkomo etsalwa kwipeptone medium enegazi lesilwanyana elingenafibrine (igazi lomvundla ngokubanzi okanye igazi legusha).Ngoko ke, ngaphezu kwezondlo ezahlukeneyo ezifunekayo ukulima ibhaktheriya, inokubonelela nge-coenzyme (njenge-factor V), i-heme (i-factor X) kunye nezinye izinto ezikhethekileyo zokukhula.Ke ngoko, indawo yenkcubeko yegazi ihlala isetyenziselwa ukulima, ukwahlukanisa kunye nokugcina iintsholongwane ezithile ze-pathogenic ezifuna isondlo.

Ukongeza, i-agar yegazi ihlala isetyenziselwa uvavanyo lwe-hemolysis.Ngexesha lenkqubo yokukhula, ezinye iibhaktheriya zinokuvelisa i-hemolysin ukuba iqhekeze kwaye ichithe iiseli ezibomvu zegazi.Xa zikhula kwipleyiti yegazi, iiringi ze-hemolytic ezicacileyo okanye eziguquguqukayo zinokubonwa malunga nekoloni.I-pathogenicity yeebhaktheriya ezininzi ihambelana neempawu ze-hemolytic.Ngenxa yokuba i-hemolysin eveliswa ziibhaktheriya ezahlukeneyo yahlukile, amandla e-hemolytic nawo ahlukile, kwaye i-hemolysis phenomenon kwi-plate yegazi nayo yahlukile.Ngoko ke, uvavanyo lwe-hemolysis luhlala lusetyenziselwa ukuchonga ibhaktheriya.

i-TCBS medium:

I-TCBS yi-thiosulfate citrate bile ityuwa ye-sucrose agar medium.Ukukhetha okukhethiweyo kwe-pathogenic vibrio.I-Peptone kunye ne-yeast extract zisetyenziswa njengezondlo ezisisiseko kwindawo yenkcubeko ukubonelela ngomthombo we-nitrogen, umthombo wekhabhoni, iivithamini kunye nezinye izinto zokukhula ezifunekayo ekukhuleni kweebhaktheriya;Uxinzelelo oluphezulu lwe-sodium chloride lunokuhlangabezana neemfuno zokukhula kwe-halophilic ye-vibrio;I-Sucrose njengomthombo wekhabhoni evundileyo;I-sodium citrate, indawo ephezulu ye-pH ye-alkaline kunye ne-sodium thiosulfate inqanda ukukhula kweebhaktheriya zamathumbu.Inkomo yenkomo engumgubo kunye ne-sodium thiosulfate inqanda ukukhula kwebhaktheriya enegram-positive.Ukongeza, i-sodium thiosulfate ikwabonelela ngomthombo wesulfure.Xa kukho i-ferric citrate, i-hydrogen sulfide inokufunyanwa yibhaktheriya.Ukuba kukho iibhaktheriya ezivelisa i-hydrogen sulfide, intlenga emnyama iya kuveliswa kwipleyiti;Iimpawu ze-TCBS eziphakathi ziyi-bromocresol eluhlaza okwesibhakabhaka kunye ne-thymol eluhlaza okwesibhakabhaka, ezizizibonakaliso zesiseko se-asidi.I-Bromocresol eluhlaza okwesibhakabhaka isalathiso se-acid-base kunye ne-pH yenguqu ye-3.8 (ephuzi) ukuya kwi-5.4 (eluhlaza okwesibhakabhaka).Kukho iindidi ezimbini ze-discoloration: (1) uluhlu lwe-asidi luyi-pH 1.2 ~ 2.8, lutshintsha ukusuka kumthubi ukuya kubomvu;(2) Uluhlu lwealkali luyi-pH 8.0 ~ 9.6, lutshintsha ukusuka kumthubi ukuya kubhlowu.

TSA itshizi yeembotyi zesoya peptone agar medium:

Ukubunjwa kwe-TSA kufana ne-agar yezondlo.Kumgangatho wesizwe, ngokuqhelekileyo kusetyenziselwa ukuvavanya iibhaktheriya zokumisa kumagumbi acocekileyo (imimandla) yoshishino lwamayeza.Khetha indawo yokuvavanya kwindawo oza kuvavanywa kuyo, vula ipleyiti ye-TSA kwaye uyibeke kwindawo yovavanyo.Iisampulu ziyakuthatyathwa xa zisemoyeni ngaphezu kwemizuzu engama-30 ngamaxesha ahlukeneyo, kwaye emva koko zikhuliswe ukubala ikoloni.Amanqanaba ahlukeneyo okucoceka afuna amanani ahlukeneyo ekholoni.

Mueller Hinton agar:

I-MH medium is medium microbial medium esetyenziselwa ukufumanisa ukuchasana kwe-microorganisms kwi-antibiotics.Yindlela engakhethiyo apho uninzi lwe-microorganisms lungakhula khona.Ukongezelela, isitashi kwizithako sinokuthatha i-toxins ekhutshwe yibhaktheriya, ngoko ayiyi kuchaphazela iziphumo zokusebenza kwe-antibiotic.Ukubunjwa kwe-MH ephakathi kukhululekile, ehambelana nokusasazwa kwe-antibiotics, ukwenzela ukuba ibonise indawo ecacileyo yokuthintela ukukhula.Kwishishini lezempilo laseTshayina, i-MH medium iphinda isetyenziswe kuvavanyo lobuzaza beziyobisi.Xa kuqhutywa uvavanyo lobuzaza kwiziyobisi kwiibhaktheriya ezikhethekileyo, ezifana neStreptococcus pneumoniae, i-5% yegazi legusha kunye ne-NAD inokongezwa kwindawo ephakathi ukuhlangabezana neemfuno ezahlukeneyo zesondlo.

I-SS agar:

I-agar ye-SS idla ngokusetyenziselwa ukuhlukaniswa okukhethiweyo kunye nenkcubeko ye-Salmonella kunye ne-Shigella.Ivimbela iibhaktheriya zegram-positive, ezininzi i-coliforms kunye neproteus, kodwa ayichaphazeli ukukhula kwe-salmonella;I-sodium thiosulfate kunye ne-ferric citrate zisetyenziselwa ukufumanisa isizukulwana se-hydrogen sulfide, okwenza i-colon center ibe mnyama;Ubomvu obungathathi hlangothi luphawu lwe-pH.I-asidi evelisa ikholoni yeswekile ebilayo ibomvu, kwaye ikoloni yeswekile engabilisi ayinambala.I-Salmonella ayinambala kwaye ikholoni ecacileyo kunye okanye ngaphandle kombindi omnyama, kwaye iShigella ayinambala kunye nekholoni ecacileyo.