Ukuhlelwa kwezitya zePetri——
1. Ngokweendlela ezahlukeneyo zokusetyenziswa kwezitya zenkcubeko, zinokwahlulwa zibe izitya zenkcubeko yeseli kunye nezitya zenkcubeko yebhaktheriya.
2. Inokwahlulwa ibe yiplastiki yepetri izitya kunye neglasi yepetri izitya ngokwezinto ezahlukeneyo zokuvelisa, kodwa uninzi lwezitya zepetri ezithunyelwa ngaphandle kunye nezitya zepetri ezilahlwayo zizinto zeplastiki.
3. Ngokutsho kobukhulu obuhlukeneyo, banokuthi bahlulwe ngokubanzi kwi-35mm, 60mm kunye ne-90mm ububanzi.150mm Petri isitya.
4. Ngokomahluko wokuhlukana, unokwahlula kwii-2 ezihlukeneyo izitya zePetri, izitya ezi-3 ezihlukeneyo zePetri, njl.
5. Izinto ezisetyenziselwa izitya zenkcubeko zihlulwe ngokwesiseko zibe ngamacandelo amabini, ngokukodwa iplastiki kunye neglasi.Iglasi ingasetyenziselwa izinto zezityalo, inkcubeko ye-microbial, kunye nenkcubeko yokubambelela kwiiseli zezilwanyana.Izinto zeplastiki zinokuba yi-polyethylene, enokusetyenziswa kanye okanye ngamaxesha amaninzi.Zilungele ukugonywa kwebhubhoratri, ukuloba, kunye nemisebenzi yokwahlula iibhaktheriya, kwaye zingasetyenziselwa ukulima izinto zezityalo.
Kutheni isitya sePetri sijonge phantsi kwinkcubeko ye-lithographic--
1. Ngethuba lokusebenza, kunokubakho amaconsi amanzi okanye ibhaktheriya kwi-cover ye-petri dish.Inkcubeko ehlanjululweyo inokuthintela ukuhla kwamanzi okanye i-microorganisms kwikhava ekuweleni kwi-petri dish.
2. Ngexesha lenkqubo yenkcubeko, iibhaktheriya ziya kuvelisa ezinye i-metabolites eziyingozi ekukhuleni nasekuzalweni kweebhaktheriya ngexesha lenkqubo yokuvelisa i-metabolic, ukukhulula ukushisa kunye nokukhupha amanzi.Ukuba iibhaktheriya azikhuliswanga phantsi, amaconsi amanzi aya kuwela kwindawo yenkcubeko, echaphazela ukukhula kweekoloni.
3. Ukuba injongo yenkcubeko kukuqokelela i-metabolites yebhaktheriya, kwaye i-metabolites i-soluble ngokulula emanzini, inkcubeko eguquliweyo ingenza lula ukuqokelela.
Ngexesha lenkcubeko, kuya kubakho umphunga wamanzi amaninzi kwisitya senkcubeko, kunye ne-condensation yomphunga wamanzi kwi-cover dish kuya kuvelisa amaconsi amanzi.Ukuba isitya senkcubeko sibekwe kwindawo efanelekileyo, amaconsi amanzi aya kusasaza iikholoni.Ngale ndlela, ikholoni enkulu inokusasazeka kwiindawo ezininzi ezincinci, ezibangela ingxaki enkulu yokulima nokubalwa kweebhaktheriya.Ukuba kubangelwa, i-culture medium iphezulu kwaye isitya siphantsi kwekhava, kwaye amaconsi amanzi awayi kuwela kwikholoni.
Izilumkiso zokusetyenziswa kwezitya zePetri——
1. Emva kokucoca kunye ne-disinfection ngaphambi kokusetyenziswa, ukucoceka kwezitya zenkcubeko kunempembelelo enkulu kumsebenzi, onokuchaphazela i-pH yenkcubeko yenkcubeko.Ukuba kukho iikhemikhali ezithile, ziya kuthintela ukukhula kweebhaktheriya.
2. Izitya zenkcubeko ezisanda kuthengwa kufuneka zihlanjwe ngamanzi ashushu kuqala, kwaye emva koko zifakwe kwi-1% okanye i-2% yesisombululo se-hydrochloric acid iiyure eziliqela ukususa izinto ze-alkaline zamahhala, kwaye zihlanjwe kabini ngamanzi adibeneyo.
3. Ukuhlakulela iibhaktheriya, sebenzisa umphunga woxinzelelo oluphezulu (ngokubanzi 6.8 * 10 Pa high pressure steam ukuya kumandla we-5th), yifake inzalo kwi-30min kwi-120 ℃, yomise kwiqondo lokushisa, okanye usebenzise ubushushu obomileyo ukwenza inzala, oko kukuthi, ukubeka. isitya senkcubeko kwi-oveni, lawula ubushushu kwi-120 ℃ nge-2h, kwaye emva koko ubulale amazinyo ebhaktheriya.
4. Kuphela izitya zenkcubeko ezihlambulukileyo zingasetyenziselwa ukugonywa kunye nokulima.
Kutheni isitya sePetri sijonge phantsi kwinkcubeko ye-lithographic--
1. Ngethuba lokusebenza, kunokubakho amaconsi amanzi okanye ibhaktheriya kwi-cover ye-petri dish.Inkcubeko ehlanjululweyo inokuthintela ukuhla kwamanzi okanye i-microorganisms kwikhava ekuweleni kwi-petri dish.
2. Ngexesha lenkqubo yenkcubeko, iibhaktheriya ziya kuvelisa ezinye i-metabolites eziyingozi ekukhuleni nasekuzalweni kweebhaktheriya ngexesha lenkqubo yokuvelisa i-metabolic, ukukhulula ukushisa kunye nokukhupha amanzi.Ukuba iibhaktheriya azikhuliswanga phantsi, amaconsi amanzi aya kuwela kwindawo yenkcubeko, echaphazela ukukhula kweekoloni.
3. Ukuba injongo yenkcubeko kukuqokelela i-metabolites yebhaktheriya, kwaye i-metabolites i-soluble ngokulula emanzini, inkcubeko eguquliweyo ingenza lula ukuqokelela.
Ngexesha lenkcubeko, kuya kubakho umphunga wamanzi amaninzi kwisitya senkcubeko, kunye ne-condensation yomphunga wamanzi kwi-cover dish kuya kuvelisa amaconsi amanzi.Ukuba isitya senkcubeko sibekwe kwindawo efanelekileyo, amaconsi amanzi aya kusasaza iikholoni.Ngale ndlela, ikholoni enkulu inokusasazeka kwiindawo ezininzi ezincinci, ezibangela ingxaki enkulu yokulima nokubalwa kweebhaktheriya.Ukuba kubangelwa, i-culture medium iphezulu kwaye isitya siphantsi kwekhava, kwaye amaconsi amanzi awayi kuwela kwikholoni.
Ixesha lokuposa: Sep-20-2022