Indlela yokusebenzisa ityhubhu ye-cryopreservation ngokwesayensi nangokuchanekileyo
Ukusetyenziswa kwetyhubhu ye-cryopreservation yinzululwazi, kungekhona i-trilogy elula efana nokuvula itanki ye-nitrogen engamanzi, uyibeke kwi-tube ye-cryopreservation kunye nokuvala itanki ye-nitrogen engamanzi.Ukusetyenziswa kwenzululwazi kunye nokuchanekileyo kweetyhubhu ze-cryopreservation kunokuphepha ukulahleka kweesampuli kunye nokukhusela ukhuseleko lwabavavanyi.
Ukusetyenziswa kwetyhubhu ye-cryopreservation yinzululwazi, kungekhona i-trilogy elula efana nokuvula itanki ye-nitrogen engamanzi, uyibeke kwi-tube ye-cryopreservation kunye nokuvala itanki ye-nitrogen engamanzi.Ukusetyenziswa kwenzululwazi kunye nokuchanekileyo kweetyhubhu ze-cryopreservation kunokuphepha ukulahleka kweesampuli kunye nokukhusela ukhuseleko lwabavavanyi.
Umbhobho wokukhenkceza: amanyathelo okukhenkceza
Geza iiseli kunye nesisombululo se-PBS esandulelayo, ufunxe isisombululo, kwaye ugubungele iiseli ngesisombululo esine-trypsin kunye ne-EDTA (ulwelo oluncinci oluncinci lwanele, kwaye ukuxinwa kwe-trypsin kunye ne-EDTA kufuneka kumiselwe ngokomgca weseli).
Fuka iiseli kwi-37 ℃ imizuzu emi-3-5.
Emva kokuba iiseli ziqhawule ukusuka ngaphantsi, i-incubation iphelile, i-medium equkethe i-serum yongezwa, kwaye iiseli zixhonywe ngobumnene nge-pipette.
I-Centrifuge i-cell suspension (500 xg, i-5 min) kunye nokumisa kwakhona kunye ne-medium equkethe i-serum.
Ubalo lweeseli.
I-Centrifuge i-cell suspension (500 xg, imizuzu emi-5), susa i-supernatant, kwaye uphinde umise iiseli kunye ne-medium equkethe i-serum yevolumu efanelekileyo.
Xuba iiseli kunye nesisombululo se-cryopreservation (i-60% ephakathi, i-20% ye-fetal bovine serum, i-20% ye-DMSO) kumlinganiselo we-1: 1 wevolumu, uze uzidlulisele kwi-Cryo STM cryopreservation tube.Ubuninzi beeseli ezifriziwe ngu-1-5 × 106 iziqwenga / ml.
I-Cryo equlethe iiseli ze-STM cryopreservation tube inconywa ukuba iphole phantsi kwinqanaba - 1 K / min, kwaye ityhubhu ye-cryopreservation ingafakwa kwisitya esine-isopropanol - 70 ℃.Ukuba i-Cryo STM cryopreservation tube igcina ezinye iisampuli, ezinokubekwa ngokuthe ngqo ku-− 20 ℃, − 70 ℃ okanye isigaba segesi senitrogen engamanzi.Ukuqinisekisa ukuba isampuli ikhenkcezwe ngokufanayo, i-4 mL kunye ne-5 mL i-Cryo Ityhubhu ye-sTM cryopreservation kufuneka ifakwe efrijini ku-− 20 ℃ ubusuku bonke, kwaye emva koko idluliselwe kwisigaba segesi - 70 ℃ okanye i-nitrogen engamanzi.
Emva koko tshintshela i-Cryo.sTM cryopreservation ityhubhu kwitanki yenitrogen engamanzi.Ukuze ugweme ukungcola (okufana ne-mycoplasma) kunye nokuqwalaselwa kokhuseleko, nceda ubeke i-Cryo.sTM cryopreservation tube kwisigaba segesi se-nitrogen engamanzi, kungekhona kwisigaba solwelo.
Indlela yokusebenzisa ityhubhu ye-cryopreservation ngokwesayensi nangokuchanekileyo?Inkampani yethu iqulunqwe ziingcali ezinemvelaphi yoshishino kunye namava emarike atyebileyo ukubonelela ngeemveliso zecandelo lophando lwesayensi yobomi kunye neenkonzo zabaphandi besayensi.Ayinakuhlangabezana kuphela neemfuno ezikhethekileyo zabathengi be-R&D kwiintlobo zemveliso kunye nokupakishwa, kodwa ikwahlangabezana neemfuno ezibanzi zamashishini emveliso kuwo onke amanqanaba ukusuka kwinqanaba elincinci, eliphakathi ukuya kwimveliso enkulu.
Ixesha lokuposa: Nov-25-2022