Ungayikhetha njani ipleyiti yePCR kwizinto ezisetyenziswa elabhoratri?
Iipleyiti ze-PCR zihlala zi-96-hole kunye ne-384-hole, zilandelwa yi-24-hole kunye ne-48-hole.Isixhobo se-PCR esisetyenzisiweyo kunye nobume besicelo esiqhubekayo siya kugqiba ukuba ibhodi ye-PCR ifanelekile kuvavanyo lwakho.Ke, ungakhetha njani ibhodi yePCR yezinto ezisetyenziswa kwilabhoratri ngokuchanekileyo?
I-1, Iindidi ezahlukeneyo zesiketi azikho iibhodi zesiketi kwaye azikho iipaneli ezijikelezileyo.
Olu hlobo lwepleyiti yokusabela lunokutshintshwa kwiimodyuli ezininzi zezixhobo ze-PCR kunye nezixhobo ze-PCR zexesha langempela, kodwa ayifanelekanga kwizicelo ezizenzekelayo.
I-half-skirt plate inemiphetho emifutshane ejikeleze umda weplate kwaye inika inkxaso eyaneleyo ngexesha lokudluliselwa kwamanzi.Uninzi lwezixhobo zePCR eziSetyenzisiweyo zeBiosystems zisebenzisa iipleyiti ezinesiqingatha seskirt.
Ibhodi ye-PCR ene-skirt epheleleyo inephaneli yomphetho evala ubude bebhodi.Olu hlobo lwebhodi lufanelekile kwisixhobo se-PCR esinemodyuli ephumayo (esilungele ukusebenza ngokuzenzekelayo), kwaye inokulungiswa ngokukhuselekileyo kunye nokuzinza.I-skirt epheleleyo iphinda ikhulise amandla omatshini, okwenza ukuba ifaneleke kakhulu ukusebenzisa iqonga lerobhothi kwindlela yokusebenza ngokuzenzekelayo.
2, Iindidi ezahlukeneyo zephaneli
Uyilo lwephaneli yeflethi epheleleyo iyasebenza kuninzi lwezixhobo zePCR kwaye ilungele ukutywinwa kunye nokusetyenzwa.
Uyilo lomphetho wepleyiti yeconvex lunokuguquguquka okuhle kwezinye izixhobo zePCR (ezifana neApplied Biosystems PCR isixhobo), enceda ukulungelelanisa uxinzelelo lwesivalo sobushushu ngaphandle kwemfuneko yeadaptha, ukuqinisekisa ukuhanjiswa kobushushu okuhle kunye neziphumo zovavanyo ezithembekileyo.
Iipleyiti ze-PCR zihlala zibonelela ngeendidi ezahlukeneyo zemibala eyahlukeneyo ukuququzelela ukwahlula okubonakalayo kunye nokuchongwa kweesampulu, ngakumbi kwimifuniselo ephezulu.Nangona umbala weplastiki ungenaso isiphumo sokukhulisa i-DNA, kucetyiswa ngakumbi ukuba kusetyenziswe izinto ezityiwayo zeplastiki ezimhlophe okanye iplastiki ekhenkcezayo enokutyiwa ekuhleni xa kumiselwa ukusabela kwexesha lokwenyani le-fluorescence quantitative PCR ukufezekisa ubhaqo olunovakalelo noluchanekileyo lwe-fluorescence.
4. Izikhundla ezahlukeneyo ze-chamfer
Ukusika ikona yikona elahlekileyo yeplate yePCR, exhomekeke kwisixhobo esiza kulungiswa.I-chamfer inokufumaneka kwi-H1, i-H12 okanye i-A12 ye-96-hole plate, okanye i-A24 ye-384-hole plate.
5, ifomathi ye-ANSI/SBS
Ukuze ihambelane neenkqubo ezahlukeneyo zokusebenzisa ulwelo oluzenzekelayo oluphathwayo oluphezulu, ibhodi ye-PCR kufuneka ihambelane ne-American National Standards Association (ANSI) kunye ne-Society for Biological and Molecular Sciences (SBS), ngoku edityaniswa ne-Laboratory Automation and Umbutho wokuHlola (SLAS).Ibhodi ehambelana ne-ANSI / SBS inobungakanani obuqhelekileyo, ubude, indawo yomngxuma, njl., eluncedo ekusebenzeni ngokuzenzekelayo.
6. Umngxuma edge
Kukho umphetho ophakamileyo ojikeleze umngxuma.Olu luyilo lunokunceda ukutywina ngefilimu yepleyiti yokutywina ukunqanda ukuvela komphunga.
7, Marko
Idla ngokuba luphawu olunyusiweyo lwealphanumeric olunombhalo wesandla omhlophe okanye omnyama kumbala wokuqala ukuze ujongwe lula.
Ixesha lokuposa: Feb-10-2023