Unokukhetha njani "ityhubhu yokukhenkceza" ebalaseleyo?

Unokukhetha njani "ityhubhu yokukhenkceza" ebalaseleyo?

Ityhubhu ye-cryo ekulula ukuyisebenzisa ayinakuhlangabezana neemfuno zovavanyo kuphela, kodwa ikwanciphisa ukubakho kweengozi zovavanyo ukuya kwinqanaba elithile.

Namhlanje siza kusebenzisa iindlela ezi-3 zokukhetha ityhubhu ye-cryo.

Inyathelo lokuqala: imathiriyeli

Njengoko sonke sisazi, iityhubhu zokukhenkceza zisetyenziswa ikakhulu kuthutho lobushushu obuphantsi kunye nokugcinwa kwezicubu okanye iisampuli zeeseli, rhoqo kuphando lwebhayoloji nakwimimandla yezonyango.

Ngenxa yokuba ityhubhu yokukhenkceza inxibelelana ngokuthe ngqo nesampulu, inyathelo lokuqala kukukhetha izinto ezifanelekileyo ukuphepha ukungcoliseka kwesampulu.

Ngokubanzi, iityhubhu zokukhenkceza zenziwe ngemathiriyeli ngaphandle kwe-cytotoxicity.Izinto eziqhele ukusetyenziswa kwiilabhoratri yiplastiki kunye neglasi.Nangona kunjalo, ngenxa yokuba ii-cryotubes zeglasi azikwazi ukusetyenziswa kwi-centrifuges enesantya esiphezulu okanye i-overspeed, ii-cryotube zeplastiki zihlala zisetyenziswa.

Kukho izinto ezininzi zeplastiki, indlela yokukhetha?

Amagama amahlanu, "i-polypropylene material" Khetha ngokuzithemba!

I-Polypropylene inemichiza egqwesileyo kunye nokuzinza kobushushu.Ngaphantsi kwemeko yegesi yenitrogen engamanzi, inokumelana nobushushu obuphantsi ukuya ku-187 ℃.

Ukongeza, ukuba iimfuno zokhuseleko lwesampulu ziphezulu kakhulu, izixhobo ezingezizo ze-mutagenic kunye ne-pyrogen yasimahla yeetyhubhu ezihambelanayo zeVID zinokukhethwa.Kwaye nceda ungayivuli phambi kokusetyenziswa.Ukuba sele ivuliwe, kufuneka icocwe phambi kokusetyenziswa!

Inyathelo lesibini: Ukuqulunqwa

Ityhubhu yokukhenkceza ngokuqhelekileyo yenziwe yityhubhu kunye netyhubhu yomzimba, eyahlulahlulwe ibe yityhubhu yokukhenkceza yangaphakathi kunye netyhubhu yokukhenkcisa yangaphandle.Ukuba isampuli iya kugcinwa kwisigaba se-nitrogen engamanzi, sebenzisa ityhubhu yokukhenkceza yangaphakathi kunye ne-silica gel pad;Ukuba isampuli iya kugcinwa kwisixhobo somatshini, njengefriji, ityhubhu yokukhenkceza yangaphandle isetyenziselwa, ngokuqhelekileyo ngaphandle kwe-silica gel pad.

Ngelizwi elinye:

Lilonke, ukuxhathisa ubushushu obuphantsi kwetyhubhu ye-cryopreservation ejikelezayo yangaphakathi ingcono kunombhobho wokukhenkceza ojikelezayo wangaphandle, omele ukhethwe ngokweemfuno zokwenyani.

Inyathelo lesithathu: iinkcukacha

Ngokweemfuno zovavanyo, iityhubhu ze-cryopreservation ngokubanzi zineenkcukacha ze-0.5ml, 1.0ml, 2.0ml, 5ml, njl.

Isampulu yebhayoloji esetyenziswa ngokuqhelekileyo ityhubhu yomkhenkce iyi-2ml ngobukhulu.Kufuneka kuqatshelwe ukuba umthamo wesampuli ngokubanzi awukwazi ukudlula isibini kwisithathu somthamo wombhobho wokuqandisa.Ngoko ke, ityhubhu efanelekileyo yokukhenkceza kufuneka ikhethwe ngokobukhulu besampulu efriziwe

Ukongeza, kukho iyantlukwano phakathi umaleko kabini kunye non-double umaleko, inokusekwa kwaye ayinakusekwa, yasekhaya kunye nokungenisa, kunye nexabiso.Ezi zizinto ekufuneka ziqwalaselwe xa ukhetha ityhubhu yokukhenkceza.