Yintoni ifilimu yokutywina?
Ifilimu yokutywina ipleyiti yifilimu yokutywina yepleyiti ebonakalayo esebenzisa ijeli, esetyenziswa ngokubanzi kuvavanyo lwe96/384 iipleyiti zequla, ezinje ngePCR, qPCR, ELISA, inkcubeko yeeseli, ukugcinwa kwexesha elide, ukusetyenzwa kwendawo yokusebenza ngokuzenzekelayo, kwaye phantse yonke imifuniselo. .Umsebenzi wayo oyintloko kukuqinisekisa ukuba ifilimu yokutywina idityaniswe ngokusondeleyo kwi-96 / 384 yeplate yomthombo ukukhusela ukuvela kwamanzi.
Kucingelwa ukuba, iintsana ezihlala zisenza olu vavanyo ziye zadibana neengxaki ezahlukeneyo ezinje nge-eddge warping, ukuphuphuma komphunga, nokukrazuka.Iimveliso ekunzima ukuzandisa ziye zaphuphuma kwisiqingatha!Intliziyo yomntu injengothuthu olufileyo;
Ukuba ufuna ukwenza umsebenzi omhle, kufuneka uqale ulole izixhobo zakho.Akulula ukufumana iimeko zovavanyo, thenga ipleyiti yePCR ekulula ukuyisebenzisa kwaye ingaguquguquki, kunye nefilimu yokutywina ipleyiti ephezulu ecacileyo.Kukwafuneka siqonde indlela echanekileyo yokuncamathisela ifilimu!
Indlela echanekileyo yokusetyenziswa kwefilimu yile ilandelayo:
Thatha ipleyiti yokutywina ifilimu okanye ipleyiti etywina ifoyile yealuminiyam kwibhegi yokuzitywina, kwaye emva koko uyivale kwakhona ibhegi yokuzitywina ukugcina i-enzayimu engenazingxaki kuyo.
▪ Bamba ifilimu yokutywina okanye ifoyile yealuminiyam yokutywina uze umphezulu womva ujonge phezulu.
Songa ileyibhile yesiphelo ezantsi kwi tangenti yokuxhasa.
▪ Ukuba imveliso esetyenzisiweyo yifilim yokutywina okanye ifoyile yealuminiyam yeleyibhile enye, susa inxalenye yephepha elixhasayo, emva koko uqhoboshele ifilimu yokutywina okanye ifoyile yealuminiyam ebhodini ukuze uyitywine kuyo yonke ibhodi, uze uqhubeke ususa ifilimu. iphepha elixhasayo.Le ndlela inokuphelisa i-curl kunye ne-rollback ebangelwa ifilimu yokutywinwa okanye i-aluminium foil.
▪ Ukuba usebenzisa imveliso enamagama amabini, colula iliner esembindini ngendlela eqhubekayo negudileyo.Kancinci ukukrazula i-liner ukuze unciphise i-curl.Qaphela ukuba ungachukumisi i-bonding surface yefilimu.
▪ Bamba amalungu amhlophe kuzo zozibini iziphelo ngezandla zozibini uze uhlise i-diaphragm kwi-orifice plate.
▪ Khuhla uze uvale ifilimu yepleyiti okanye ifoyile yealuminiyam kancinane ngokucinezela ipleyiti ukuze ivale ipleyiti.Eli nyathelo kufuneka lenziwe ubuncinane kabini ngokuthe tye kwaye ngokuthe nkqo.Ukusebenzisa amandla awoneleyo kubalulekile ukuze ufumane ukutywinwa okulungileyo.(jonga isazobe sendlela yokutywina engezantsi):
▪ Khupha kwaye ucinezele ipleyiti ubuncinane kabini ecaleni kwayo yonke imiphetho yangaphandle yepleyiti ephuma emlonyeni ukuqinisekisa ngoxinzelelo oluqinileyo noluqhubekayo.
▪ Emva kokutywinwa, jonga ipleyiti ecaba ukuqinisekisa ukuba ifilim / ifoyile ibotshelelwe ngokuqinileyo kwipleyiti.Ifilimu yokutywina okanye i-aluminium foil akufanele ibe nemibimbi.Ukuba imibimbi iyabonwa, ibonisa ukuba ipleyiti ayivalwanga kakuhle.Kufuneka kwakhona kuqatshelwe ukuba ifilimu yokutywina okanye i-aluminium foil ayifanele inyukele phezulu kudonga olusecaleni lweplate.Kwiipleyiti ezisicaba ezinemiphetho ephakanyisiweyo, oku kunokwenzeka ngenxa yokuba ifilimu yokutywina okanye ifoyile yealuminiyam ayibekwanga ngokuchanekileyo kwipleyiti, okanye amakhonkco kwiziphelo zombini azikrazulwa.Qinisekisa amanqaku okunamathisela ajikeleze umngxuma ngamnye, kwaye yonke indawo yeplate (kubandakanywa ne-periphery) ivaliwe.
▪ Emva kokuba ifilimu yokutywina okanye ifoyile yealuminiyam ivalwe kakuhle ebhodini, qhawula umdibaniso omhlophe kuzo zombini iziphelo ecaleni kwetangenti.(isiphumo sibonisiwe kulo mfanekiso ungezantsi):
▪ Kungcono ukushiya ipleyiti etywiniweyo ubuncinane imizuzu eyi-10 ngaphambi kokuba uqalise uvavanyo lwe-PCR, kwaye ukunamathela kwefilimu yokutywina kuya kwanda ngokuhamba kwexesha.
▪ Dlulisa ipleyiti kumatshini wePCR kwaye usebenzise umatshini wePCR.
Kukho iintlobo ezininzi zeefilimu zokutywina iipleyiti zeLabio, ezinokubonelela phantse zonke iintlobo zeefilimu zokutywina kwizicelo zovavanyo kubasebenzisi, kwaye zinokusetyenziswa kwizicelo ezininzi ezibandakanya iPCR, qPCR, ELISA, inkcubeko yeseli, ukugcinwa kwexesha elide, iautomated. ukulungiswa kwendawo yokusebenza, njl.
Ixesha lokuposa: Sep-08-2022