Indlela yokukhetha ngokuchanekileyo ukusetyenziswa kwenkcubeko yeseli "iiflaski, iiplate kunye nezitya"?

Indlela yokukhetha ngokuchanekileyo ukusetyenziswa kwenkcubeko yeseli "iiflaski, iiplate kunye nezitya"?

Xa uhlakulela iiseli, ixesha lokusebenzisa iiflaski zenkcubeko kunye nexesha lokusebenzisa iipleyiti zequla kuxhomekeke kwinjongo kunye neemfuno zovavanyo.Ngokubanzi, iiflasks zenkcubeko yeeseli zisetyenziselwa inkcubeko yeseli esisiseko kunye ne-subculture eqhelekileyo, kwaye inani elikhulu leeseli zovavanyo zinokufunyanwa.

Iiflask zenkcubeko yeeseli zenziwe nge-polystyrene (PS) ekumgangatho ophezulu wezinto, eziveliswe ngokubumba kwe-ultra-precision kunye neenkqubo zokuvelisa ezizenzekelayo ngokupheleleyo.Iimveliso zisetyenziselwa inkcubeko yeseli yebhubhoratri.Iimpawu zabo ezibalaseleyo zamehlo ziququzelela ukubonwa kwe-microscopic.Umphezulu uye waphathwa nge-TC ukuqinisekisa ukunamathela kwiseli.Iziphumo ezingcono.

1) Ungazikhetha njani iiflaski zenkcubeko kunye neepleyiti zenkcubeko kwiiseli zenkcubeko

Okokuqala, khetha ngokusekelwe kwisivuno seseli esilindelekileyo.

Okwesibini, khetha ngokusekelwe kubuchule bemisebenzi yovavanyo.Nokuba itshintsha imidiya, idlula, okanye iiseli zokuvuna, ukusebenza kwezitya zenkcubeko kulula ngakumbi, kodwa ngenxa yokuvulwa kwayo okukhulu, kulula ukungcolisa.

I-2) Kungcono ukusebenzisa iipleyiti zenkcubeko yeeseli kwiimvavanyo ezisebenzisa iiseli njengabathwali okanye izinto, ezifana nokuvavanywa kweziyobisi, i-MTT (i-96-well culture plate), i-immunohistochemistry (i-6-well culture plate), njl.