Ngaba ufundile umgangatho wefilimu yokutywina?
Intoni?Ngubani omnye ongakwaziyo “ukutywina ifilimu”?Khawuleza uxhalabele eli nqaku ukuze likufundise "ifilimu yokutywina" echanekileyo!
Ngokuqinisekileyo, "ifilimu yokutywina" apha kukutywina ipleyiti ye-PCR engama-96 ukuqinisekisa ukuba ifilimu yokutywina ihambelana ngokusondeleyo nepleyiti yomngxuma engama-96 kwaye ithintele ukuvela kolwelo, ukuze kuqinisekiswe uvavanyo olugudileyo.
1. Ncamathelisa ifilimu yokutywina ebhodini
Thatha i-membrane yokutywina enye kwibhegi yokuzitywina, kwaye emva koko uvale ibhegi yokuzitywina ukuze ugcine i-enzayim ingenandawo.Gcina umgca ongezantsi ujonge phezulu, ubambe ifilimu yokutywina, kwaye ngokukhawuleza ukrazule umgca ongezantsi kunye nomgca we-tangent.
Emva koko, unamathele enye isiphelo somgangatho wokunamathela wefilimu yokutywina ebhodini, kwaye ubambe umgama kunye ne-angle ukuze ugweme i-skew elandelayo.Kwinkqubo yokuncamathisela, isiphelo sincamatheliswa kwaye esinye siyatsalwa.
Icebiso: Hlala unxibe iiglavu
● Ukuba ifilimu yokutywina yeleyibhile enye isetyenzisiweyo, susa i-liner ngokuyinxenye, qinisa ifilimu yokutywina ebhodini ukuze uyitywine kwibhodi yonke, uze uqhubeke nokususa i-liner.Le ndlela inokuphelisa i-curl kunye ne-rollback ebangelwa yifilimu yokutywinwa.
● Ukuba imveliso eneelebhile ezimbini isetyenzisiwe, hlambulula i-liner ephakathi ngendlela eqhubekayo kwaye egudileyo.Ukuhluthwa okucothayo kwelayini kunciphisa ukukrala.Qaphela ukuba ungachukumisi i-bonding surface yefilimu.
2. Ukucofa ifilimu
Sebenzisa ipleyiti yoxinzelelo ukukrazula kancinci kwaye ucinezele ifilimu yepleyiti yokutywina ukuze ivalwe ngokupheleleyo kwipleyiti.Ukuba akukho laminates ezikhethekileyo, unokufumana ikhadi elinemiphetho egudileyo, njengekhadi lebhanki okanye ikhadi lebhasi.
Inyathelo lokucinezela ifilimu liya kwenziwa ubuncinane kabini ngokuthe tye nathe nkqo.Kubalulekile ukusebenzisa amandla aneleyo ukufumana isitywina esilungileyo.
Khupha kwaye ucinezele i-membrane yokucinezela ipleyiti ecaleni kwayo yonke imiphetho yangaphandle yeplate ye-orifice ubuncinane kabini ukuqinisekisa ukuba uxinzelelo oluqinileyo noluqhubekayo luyasetyenziswa.Imingxuma kunye nemiphetho iya kucinezelwa kube kanye.Emva kokutywina ifilimu yokutywina ngokuchanekileyo kwi-plate, khupha icandelo elidibeneyo kunye nomgca we-tangent.
Ingcebiso: ● Xa ucofa ifilim, bamba ibhodi ngesinye isandla ukunqanda ukushukunyiswa ngamandla kwebhodi.
3. Ukuhlolwa
Emva kokutywinwa, jonga ngokucophelela i-flat plate ukuze uqinisekise ukuba ifilimu ifakwe ngokusondeleyo kwi-plate.Qinisekisa ukuba amanqaku okunamathela ajikeleze umngxuma ngamnye, yonke indawo yepleyiti (kubandakanya i-periphery) itywinwe, kwaye ingaba kukho ulwelo kwi-membrane.Ifilimu yokutywina akufanele ibe nemibimbi.Ukuba imibimbi iyabonwa, ipleyiti ayivalwanga ngokuchanekileyo.
● Kwiipleyiti ezisicaba ezinemiphetho ephakanyisiweyo, ukubekwa kwefilimu yokutywina kwipleyiti isenokungachaneki, kwaye ifilimu ayisayi kunyukela phezulu kudonga olusecaleni lwepleyiti.
Beka ipleyiti etywiniweyo ubuncinane imizuzu eyi-10 ngaphambi kokuba uqalise uvavanyo lwe-PCR, kwaye i-adhesive force yefilimu yokutywinwa iya kwanda ngexesha.Ukuba kuyenzeka, sebenzisa i-centrifuge ekhethekileyo ye-orifice plate ukwenzela i-centrifugation.Okokugqibela, tshintshela ipleyiti etywiniweyo kumatshini wePCR ukuqalisa umfuniselo ~
Ingcebiso:
● Kwiipleyiti ezisicaba ezinemiphetho ephakanyisiweyo, ukubekwa kwefilimu yokutywina kwipleyiti isenokungachaneki, kwaye ifilimu ayisayi kunyukela phezulu kudonga olusecaleni lwepleyiti.
Beka ipleyiti etywiniweyo ubuncinane imizuzu eyi-10 ngaphambi kokuba uqalise uvavanyo lwe-PCR, kwaye i-adhesive force yefilimu yokutywinwa iya kwanda ngexesha.Ukuba kuyenzeka, sebenzisa i-centrifuge ekhethekileyo ye-orifice plate ukwenzela i-centrifugation.Okokugqibela, tshintshela ipleyiti etywiniweyo kumatshini wePCR ukuqalisa umfuniselo ~
Ixesha lokuposa: Dec-16-2022