Ukukhuliswa kweeseli bubuchwephesha bovavanyo obubaluleke kakhulu kwaye iye yaba yindlela yophando eyimfuneko kwiinkalo ze-biopharmaceutics, isayensi yobomi, ukutshintshwa kwekliniki, njl.Iibhotile zenkcubeko yeeseli kunye nezitya zenkcubeko ziintlobo ezimbini eziqhelekileyo.Yintoni umahluko phakathi kwezi zinto zimbini zisetyenziswayo?
Ibhotile yenkcubeko yeeseli ifanelekile kwinkcubeko yexesha elide kunye nokuhamba njengeeseli zembewu.Umlomo webhotile uncinci kwaye iiseli azilulanga ukungcoliswa.Izitya zenkcubeko yeseli zifanelekile kwinkcubeko yesikhashana kwiimvavanyo ezahlukeneyo.Umahluko phakathi kwezi zimbini ulele kwinkalo yokhuseleko kunye nenani leeseli ezikhuliswayo.Isitya sokulinga inkcubeko kunye neeseli njengomphathi okanye into ingcono, kuba inani elisetyenzisiweyo lincinci, iiseli zigcinwa, kwaye isitya senkcubeko sikulungele ngakumbi kuvavanyo lokulawula, kodwa ukuvulwa kwesitya senkcubeko kukhulu, okungaphezulu. kungenzeka ukuba ungcoliseke, ngoko kufuneka ulumke ngakumbi xa usebenza.
I-flask yenkcubeko isetyenziselwa inkcubeko yokuqala ye-tissue block okanye inkcubeko yeeseli ezingcolisekileyo ngokulula.Emva kokuba iiseli zenziwe zangaphantsi, zinokumiselwa ngokokhetho lomntu.Indawo yebhotile yenkcubeko yeseli inkulu, ngoko ke ibhotile yenkcubeko ingasetyenziselwa xa inani elikhulu leeseli lifuna ukwandiswa.
Isitya sokukhuliswa kweeseli kunye nezitya zenkcubeko zizikhongozeli ezisetyenziselwa i-microbial okanye inkcubeko yeseli elabhoratri.Uhlobo oluthile lwezinto ezisetyenziswayo eziza kusetyenziswa zixhomekeke kwiimfuno ezithile zovavanyo, kwaye kwakhona ithathela ingqalelo imo yenkcubeko yeseli, nokuba yinkcubeko yokunqunyanyiswa okanye inkcubeko ebambelelayo.Izinto ezisetyenziswayo ezifanelekileyo zisisiseko sempumelelo yovavanyo.
Ukuze ufunde ngakumbi malunga nezinto ezisetyenziswayo zovavanyo, nceda ulandele iwebhusayithi yethu.ILabio iya kuqhubeka ikubonelela ngovavanyo lwamva nje lokubonisana noncedo.
Ixesha lokuposa: Sep-28-2022