Indlela yokusetyenziswa ngokuchanekileyo kunye namanyathelo epayipi ye-serological

I-pipette ye-serological, eyaziwa ngokuba yi-pipette elahlayo, isetyenziselwa ukulinganisa ngokuchanekileyo umthamo othile wolwelo, omele usetyenziswe kunye nepayipi efanelekileyo.I-Pipette sisixhobo sokulinganisa esisetyenziselwa ukudlulisa ngokuchanekileyo umthamo othile wesisombululo.I-pipette sisixhobo sokulinganisa, esisetyenziselwa kuphela ukulinganisa umthamo wesisombululo esikhuphayo.Yityhubhu yeglasi ende necekethekileyo enokwandiswa okukhulu phakathi.Isiphelo salo esisezantsi sikwimo yomlomo obukhali, kwaye intamo yombhobho ephezulu ibhalwe ngomgca wokumakisha, owuphawu lomthamo ochanekileyo oza kuhanjiswa.

Indlela yokusetyenziswa ngokuchanekileyo kunye namanyathelo e-serum pipette:

1. Ngaphambi kokusetyenziswa: Xa usebenzisa i-pipette, jonga kuqala uphawu lwe-pipette, inqanaba lokuchaneka, indawo yokulinganisa isikali, njl.

2. Ukuphefumula: Bamba isiphelo esiphezulu se-pipette ngesithupha kunye nomnwe ophakathi wesandla sakho sokunene, kwaye ufake umlomo ongezantsi we-pipette kwisisombululo sokuba ufunxe.Ufakelo akufuneki lube nzulu kakhulu okanye lube nzulu kakhulu, luqhele ukuba yi-10 ~ 20mm.Ukuba ayinzulu kakhulu, iya kubangela ukufunxa.I-Aspiration yesisombululo kwibhola yokuhlamba indlebe iya kungcolisa isisombululo.Ukuba inzulu kakhulu, iya kubamba isisombululo esikhulu ngaphandle kombhobho.Thatha ibhola yokuhlamba indlebe ngesandla sasekhohlo, uyidibanise kumlomo ophezulu wombhobho kwaye ngokukhawuleza uphefumlele isisombululo.Okokuqala phefumlela malunga ne-1/3 yomthamo wombhobho.Cinezela umlomo wetyhubhu ngomnwe wesalathisi sesandla sokunene, yikhuphe, uyibambe ngokuthe tye, kwaye ujikeleze ityhubhu ukwenza isisombululo siqhagamshelane nenxalenye engaphezulu kwesikali sokubuyisela amanzi kudonga lwangaphakathi.Emva koko ukhuphe isisombululo emlonyeni ongezantsi wombhobho kwaye uyilahle.Emva kokuhlamba ngokuphindaphindiweyo kathathu, unokuthatha isisombululo malunga ne-5mm ngaphezu kwesikali.Ngokukhawuleza cinezela umlomo wombhobho ngomnwe wesalathisi sesandla sokunene.

3. Lungisa inqanaba le-liquid: phakamisa i-pipette phezulu kwaye usuke kwinqanaba le-liquid, sula ulwelo olukudonga lwangaphandle lwe-pipette ngephepha lokucoca, isiphelo sombhobho sixhomekeke eludongeni lwangaphakathi lwesitya sesisombululo, ityhubhu. umzimba uhlala nkqo, phumla kancinci umnwe wesalathisi ukwenza isisombululo kwityhubhu siphume kancinci siphuma kumlomo ongezantsi, kude kube sezantsi kwe-meniscus yesisombululo sijonge ukumakisha, kwaye kwangoko ucinezele umlomo wombhobho ngomnwe wesalathisi.Susa i-drop drop drop eludongeni, yisuse kwi-pipette, kwaye uyifake kwisitya esifumana isisombululo.

4. Ukukhutshwa kwesisombululo: Ukuba isitya sokufumana isisombululo yi-flask ye-conical, i-flask ye-conical kufuneka ifakwe kwi-30 °.I-pipette elahlayo kufuneka ibe nkqo.Isiphelo esisezantsi sombhobho kufuneka sibe kufuphi nodonga lwangaphakathi lwe-conical flask.Khulula umnwe wesalathisi kwaye uvumele isisombululo sehle kancinci ngodonga lwebhotile.Xa inqanaba le-liquid liwela kwintloko yokukhupha, ityhubhu idibanisa nodonga lwangaphakathi lwebhotile malunga nemizuzwana eyi-15, kwaye emva koko isuse i-pipette.Isixa esincinci sesisombululo esisele ekupheleni kombhobho akufanele sinyanzeliswe ukuba siphume, Ngenxa yokuba umthamo wesisombululo esigciniweyo ekupheleni kuthathelwe ingqalelo.