Iimpawu eziqhelekileyo zeflask yenkcubeko yeseli

Iimpawu eziqhelekileyo zeflask yenkcubeko yeseli

Inkcubeko yeseli ibhekisa kwindlela elinganisa ubume obungaphakathi kwi-vitro ukuyenza iphile, ikhule, ivelise kwakhona kwaye igcine ubume bayo obuphambili kunye nomsebenzi.Iintlobo ngeentlobo zezinto ezisetyenziswa kwinkcubeko yeeseli ziyafuneka kwinkcubeko yeseli, apho ibhotile yenkcubeko yeseli yeyona isetyenziswa kakhulu.

Ubume beflask yenkcubeko yeseli sisikwere kwaye ibhotile ibanzi.Olu yilo kukwenza lula ukuvunwa kweeseli.Icala leflask lidla ngokuqhwala, elilungele ukuba umqhubi abhale phantsi.Ngokubhekiselele kwiinkcukacha, izinto eziqhelekileyo ezisetyenziswayo ziyi-25cm2, 75cm2, 175cm2, 225cm2, njl.Kukho iimeko ezahlukeneyo zokusetyenziswa ngokweenkcukacha ezahlukeneyo.

Iibhotile ezincinci ze-25cm2 kunye ne-75cm2 zisetyenziselwa ukubuyiswa kweeseli kunye nokwandiswa okuncinci, ngokukodwa kwinqanaba lokuqala lenkcubeko yeseli.Isenokusetyenziswa njengendawo yokugcina iiseli ezibomvu zegazi ezahlukeneyo.Iibhotile ezinkulu ze-175 cm2 kunye ne-225 cm2 zisetyenziswa ikakhulu kwisikali esiphakathi senkcubeko yeseli okanye iprotein ye-eukaryotic.Inkcazo yeprotheyini yitekhnoloji yebhayoloji yemolekyuli esebenzisa ibhaktheriya, igwele, iiseli zezinambuzane, iiseli ezanyisayo okanye iiseli zezityalo ukubonisa iiproteni zemfuza zangaphandle, kwaye yenye yezinto ezibalulekileyo zobuchwephesha bobunjineli bemfuza.

Iflask yenkcubeko yeeseli isetyenziswa ikakhulu kwinkcubeko yeeseli ezibambelelayo.Nangona iinkcukacha ezahlukeneyo ziyahluka ngokusetyenziswa, kufuneka zihlangabezane neemfuno zokukhula kweeseli, ezifana ne-DNase, akukho enzyme ye-RNA, akukho endotoxin, akukho mthombo wezilwanyana, unyango lwe-TC lwangaphandle, njl.

  Iflask yenkcubeko yeseli yeLabio inezi mpawu zilandelayo:

1.Yenziwe ngezinto ezinyulu zenqanaba lezonyango i-polystyrene (PS), ebonisa ukungafihlisi okuphezulu kunye nokumelana neekhemikhali ezilungileyo.

2.Ifaka umphezulu ogudileyo kunye noyilo olufanayo lwe-cap, kunye ne-0.22 μm isihluzo se-hydrophobic ukutshintshiselana lula

I-3.Intamo ye-sloping evumela ukusebenza lula kwe-spatula yeseli kunye ne-pipettors

4.Ngendawo ebhaliweyo eneqabaka entanyeni, kunye nezidanga kumacala omabini i-ultrasonic welding esetyenziswa phakathi kwesigqubuthelo kunye neflaski, ukuthintela ukuvuza kunye nesinyithi kwi-adhesive.

Uyilo lwe-5.Stackable yokugcina indawo kunye nokugcinwa okulula

6.Yenziwe kwigumbi elicocekileyo le-100,000, eline-DNase, i-RNase, i-pyrogen kunye ne-endotoxic

7.Ipakishwe ngokuzimeleyo, inzalo ngokukhanya, i-SAL 10-6

8.Ifumaneka ngeendlela ezintathu, i-TC iphathwe okanye ayiphathwa ukuba ikhethe kuyo.