Ityhubhu ye-centrifuge yityhubhu elula enokumelana nesantya esiphezulu sokujikeleza kunye noxinzelelo, njengokwahlula iisampulu ezithile kunye nokwahlula intlenga engaphezulu.I-tube ye-ultrafiltration ye-centrifuge inamalungu amabini afana ne-tube yangaphakathi kunye ne-tube yangaphandle.Umbhobho wangaphakathi yinwebu enobunzima obuthile bemolekyuli.Ngexesha le-high-speed centrifugation, abo banobunzima obuncinane bemolekyuli baya kuvuza kwityhubhu esezantsi (okt kumbhobho wangaphandle), kwaye abo banobunzima bemolekyuli obukhulu baya kubanjwa kumbhobho ongaphezulu (okt kumbhobho ongaphakathi).Lo mgaqo we-ultrafiltration kwaye uhlala usetyenziselwa ukugxila kwiisampuli.
Iityhubhu ze-Ultrafiltration ze-centrifuge zidla ngokusetyenziswa ngaphandle kokunyangwa kwangaphambili, kodwa kwiprotheni yesampula yokucubungula, ngokukodwa kwiisombululo zeprotheyini edibeneyo (< 10ug / ml), izinga lokubuyisela ukugxila kunye ne-ultrafiltration membranes kaninzi ayinalo ubuninzi.Nangona imathiriyeli ye-PES inciphisa ukufakwa kwe-nonspecific adsorption, ezinye iiproteni, ngakumbi xa zixutywe, zinokuba neengxaki.Iqondo lokubophelela okungachazwanga liyahluka ngokwesakhiwo seprotheni nganye.Iiprotheyini eziqulathe iindawo ezihlawuliswayo okanye ezine-hydrophobic zikholisa ukubophelela ngokungaguqukiyo kwimigangatho eyahlukeneyo.I-Passivation pretreatment kumphezulu we-ultrafiltration centrifuge tube inokunciphisa ilahleko yeprotein adsorption kumphezulu we-membrane.Kwiimeko ezininzi, ukulungiswa kwangaphambili kwekholomu ngaphambi kokugxininisa isisombululo seprotheni edibeneyo kunokuphucula izinga lokubuyisela, kuba isisombululo sinokuzalisa iindawo ezingenanto ze-protein adsorption ezivezwe kwi-membrane kunye nomphezulu.Indlela yokugqithisa kukucwina kwangaphambili ikholamu kunye nomthamo ophezulu wesisombululo se-passivation ngaphezu kweyure eli-1, uhlambe ikholamu ngokucokisekileyo ngamanzi adityanisiweyo, kwaye emva koko uyibeke nge-centrifuge kube kanye ngamanzi adityanisiweyo ukususa ngokupheleleyo isisombululo se-passivation esinokuthi sihlale kwifilimu. .Qaphela ukuba ungavumeli ifilimu yome emva kokugqithisa.Ukuba ufuna ukuyisebenzisa kamva, kufuneka ungeze amanzi angcolileyo adibeneyo ukuze ugcine ifilimu ifumile.
Iityhubhu ze-Ultrafiltration centrifuge azinakwenzeka ukuvalelwa kwaye ziphinde zisetyenziswe.Ekubeni ixabiso letyhubhu enye ayibizi, abantu abaninzi bazama ukuphinda bayisebenzise - amava kukucoca umphezulu we-membrane ngamanzi adibeneyo ngamaxesha amaninzi kunye ne-centrifuge kanye okanye kabini.Ityhubhu encinci enokuthi ifakwe i-centrifuged in reverse inokuntywiliselwa emanzini adityanisiweyo kwaye emva koko i-centrifuged in reverse ngamanye amaxesha, okuya kuba ngcono.Ingasetyenziselwa isampuli efanayo ngokuphindaphindiweyo, kwaye inokucwiliswa emanzini adibeneyo xa ingasetyenziswanga, kodwa ukungcoliswa kwebhaktheriya kuya kuthintelwa.Musa ukuxuba iisampulu ezahlukeneyo.Abanye abantu bathi ukucwina kwi-20% yotywala kunye ne-1n NaOH (i-sodium hydroxide) inokuthintela ukukhula kwebhaktheriya kunye nokuthintela ukomisa.Ngethuba nje i-membrane ye-ultrafiltration ihlasela amanzi, ayinakuvunyelwa ukuba yome.Nangona kunjalo, abanye bathi iya kutshabalalisa ukwakheka kwenwebu.Kuyo nayiphi na imeko, abavelisi ngokubanzi abaxhasi ukusetyenziswa kwakhona.Ukusetyenziswa okuphindaphindiweyo kuya kuvala ubungakanani bepore ye-membrane yokucoca, kwaye kubangele ukuvuza kolwelo, okuya kuchaphazela iziphumo zovavanyo.
Ixesha lokuposa: Sep-05-2022