Iingcebiso ezi-3 zokukhetha izinto ezinokusetyenziswa kwinkcubeko yeseli
1. Misela indlela yokulima
Ngokweendlela ezahlukeneyo zokukhula, iiseli zinokwahlulwa zibe ziiseli ezibambelelayo kunye neeseli ezixhonyiweyo, kwaye kukho neeseli ezinokukhula zibambene okanye zinqunyanyiswe, njengeeseli zeSF9.Iiseli ezahlukeneyo nazo zineemfuno ezahlukeneyo zokusebenzisa inkcubeko yeeseli.Iiseli ezibambelelayo ngokubanzi zisebenzisa izinto ezityiwayo eziphathwa nge-TC, ngelixa iiseli ezinqunyanyisiweyo azinazo iimfuno ezinjalo, kodwa izinto ezisetyenziswa yi-TC-treatment nazo zifanelekile ukukhula kweeseli ezinqunyanyisiweyo.Ukukhetha izinto ezisetyenziswayo ezifanelekileyo, okokuqala kuzo zonke, misela imowudi yenkcubeko yeseli ngokohlobo lweeseli.
2. Khetha uhlobo olusebenzisekayo
Izinto eziqhelekileyo ezisetyenziswa kwinkcubeko yeseli ziquka ipleyiti yenkcubeko yeseli, ibhotile yenkcubeko, isitya senkcubeko, i-triangular shake flask, i-pipette, njl. Ezi zinto zisetyenziswayo zineempawu ezahlukeneyo kwindawo yenkcubeko, indlela yokusetyenziswa, isakhiwo ngokubanzi, njl. Ibhotile yenkcubeko yeyenkcubeko evaliweyo, enokuthi ukunciphisa ungcoliseko;Ipleyiti yenkcubeko kunye nesitya senkcubeko yeyenkcubeko evulekileyo, elungele ukulinga ulawulo kunye novavanyo lwegradient, kodwa kulula ukuzisa ungcoliseko lwebhaktiriya, olufuna iimfuno eziphezulu zabaqhubi.
Ngamafutshane, xa ukhetha uhlobo lwezinto ezisetyenziswayo, kufuneka sithathele ingqalelo iimfuno zovavanyo kunye nezinto ezikhethwayo zokusebenza komntu.
3. Iinkcukacha ezikhethiweyo ezinokusetyenziswa
Imifuniselo yenkcubeko yeeseli ezinkulu ifuna izinto ezinokutyiwa ezinommandla omkhulu wenkcubeko njengenkxaso, ngelixa imifuniselo emincinci ikhetha izinto ezisetyenziswayo ezinommandla omncinci.Iifektri zeeseli zisetyenziselwa ubukhulu becala inkcubeko yeeseli, ezifana nokuveliswa kwesitofu sokugonya, i-monoclonal antibody, ishishini lamayeza, njl.Ipleyiti yenkcubeko, isitya senkcubeko kunye nebhotile yenkcubeko zilungele inkcubeko yeseli encinci kwilabhoratri;Ukongeza kwinkcubeko yeseli yokunqunyanyiswa, i-flask engunxantathu ingasetyenziselwa ukulungiswa, ukuxuba kunye nokugcinwa kwendawo yenkcubeko.Qinisekisa iinkcukacha ezithile zezinto ezisetyenziswayo ngokomlinganiselo wenkcubeko yeeseli.
Izinto ezifanelekileyo zokusebenzisa inkcubeko yeeseli zisisiseko sokuqinisekisa ukukhula okuhle kweeseli, kunye nesitshixo sokukhawulezisa inkqubo yovavanyo kunye nokuqinisekisa isiphumo senkcubeko.Ukhetho kufuneka luthathele ingqalelo imiba efana nemo yenkcubeko yeeseli, isikali senkcubeko kunye neemeko zelabhoratri.
Ewe kunjalo, kubalulekile ukukhetha iqonga elineemveliso ezahlukahlukeneyo, unikezelo oluzinzileyo lwempahla, umgangatho oqinisekisiweyo kunye neenkonzo, ezifana neLabio, iLabio inokubonelela ngeenkonzo ezibanzi zokuthengwa kwempahla enye yokubonelela ngophando lwezenzululwazi kwiilabhoratri kwiinkalo zobomi behlabathi. isayensi, ishishini lamachiza, ukukhuselwa kokusingqongileyo, ukhuseleko lokutya, ii-arhente zikarhulumente kunye namayeza eklinikhi.
Ixesha lokuposa: Feb-09-2023