Ungayikhetha njani ipleyiti ye-ELISA efanelekileyo?

Ungayikhetha njani ipleyiti ye-ELISA efanelekileyo?

Ubume bomzantsi
Umzantsi oMcaba: Umzantsi uxwesileyo, ukwabizwa ngokuba ngu-F ezantsi.Ukukhanya okudlula emazantsi akuyi kuphambukiswa, kwaye ukuhanjiswa kokukhanya kunokwandiswa.Isetyenziselwa imifuniselo efuna umzantsi ojikelezayo wokubonakala okanye ezinye izizathu.
Umzantsi ojikelezayo: Ekwaziwa ngokuba yi-U-bottom, ibonelela ngokucocwa ngokugqibeleleyo kunye nokusebenza kokuxuba izicelo ezifuna ukuvavanywa kwentlenga.
C-Ezantsi: Phakathi komzantsi osicaba kunye nomzantsi ongqukuva obonelela ngeziphumo ezilungileyo zokucoca kunye nokudibanisa okuhle komzantsi osicaba.
Ikhowuni esezantsi: Ikwabizwa ngokuba yi-V ezantsi, ifanelekile iisampulu ezichanekileyo kunye nokugcinwa kweesampulu ezincinci zokufumana kwakhona imiqulu emincinci.
Umbala
Uninzi lwee-ELISAs zikhetha iipleyiti ezicacileyo njengemathiriyeli yovavanyo.Iipleyiti ezimhlophe kunye nezimnyama zisetyenziselwa ukubonwa kwe-luminescence.Iipleyiti ze-ELISA ezimnyama zinokukhanya kwazo, ngoko ke umqondiso wazo ubuthathaka kuneepleyiti ze-ELISA ezimhlophe.Iipleyiti ezimnyama ngokuqhelekileyo zisetyenziselwa ukukhangela ukukhanya okunamandla, okufana nokubonwa kwe-fluorescence;ngokuchaseneyo, iipleyiti ezimhlophe zingasetyenziselwa ukukhangela ukukhanya okubuthathaka, kwaye ziqhele ukusetyenziselwa i-chemiluminescence jikelele kunye nophuhliso lombala we-substrate (umz. uhlalutyo lwegene ye-dual-luciferase reporter).
Izinto eziphathekayo
Izinto eziqhelekileyo ziyi-polyethylene, i-PE, i-polypropylene, i-PP, i-Polystyrene, i-PS, i-Polyvinylchloride, i-PVC, i-Polycarbonate, i-PC.
Izinto ezisetyenziswa kakhulu kwi-ELSIA yi-polystyrene kunye ne-polyvinyl chloride.I-Polyvinyl chloride ithambile, incinci, iyasikwa kwaye ayibizi.Ukungalungi kukuba ukugqiba akulungile njengamaphepha e-polystyrene kwaye umzantsi womngxuma awukho tyaba njengepolystyrene.Nangona kunjalo, kukho ukunyuka okuhambelanayo kumaxabiso angasemva.Ngokuqhelekileyo, umphezulu wepleyiti yokubhala i-enzyme kufuneka uphathwe ngokuxhunyelelwa kwe-ionic, ekwazisa amaqela asebenzayo asebenzayo njengeqela le-aldehyde, iqela le-amino kunye neqela le-epoxy kumphezulu we-polymer ukuphucula ukusebenza komphezulu we-substrate.
Iindlela ezahlukeneyo zokubopha
Ukubophelela okusebenzayo kwento egqunyiweyo ukuya ezantsi